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master's thesis
Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae
Zagreb: University of Zagreb, Faculty of Science, 2016. urn:nbn:hr:217:417404
Busch, Franka
University of Zagreb
Faculty of Science
Department of Biology

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Busch, F. (2016). Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:417404

Busch, Franka. "Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:417404

Busch, Franka. "Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae." Master's thesis, University of Zagreb, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:217:417404

Busch, F. (2016). 'Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae', Master's thesis, University of Zagreb, Faculty of Science, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:417404

Busch F. Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2016 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:217:417404

F. Busch, "Analiza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:217:417404

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Metadata
TitleAnaliza uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae
AuthorFranka Busch
MentorJasna Hrenović (mentor)
Committee memberJasna Hrenović (predsjednik povjerenstva)
Committee memberPetar Kružić (član povjerenstva)
Committee memberAna Galov (član povjerenstva)
Committee memberRenata Matoničkin Kepčija (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2016-02-29, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
U istraživanju je provedeno ispitivanje uloge međustanične komunikacije u simbiozi Rhizobium-Leguminosae u svrhu boljeg razumijevanja međustanične komunikacije u procesu fiksacije dušika. Metodama molekularnog inženjerstva konstruirani su mutanti cinR i traI gena koji su dio genoma bakterije soja Rhizobium leguminosarum bv. viciae UPM791. Geni cinR i traI su izabrani iz razloga što imaju kontrolu nad acil-homoserin lakton ovisnom sistemu međustanične komunikacije. Mutanti gena cinR i traI su uspješno dobiveni i podvrgnuti daljnjim istraživanjem utjecaja mutanata na interakciju s biljkama. Preliminarni rezultati dobiveni TLC analizom ukazuju da cinR mutant smanjeno proizvodi C14-HSL molekule kao i kratkolančane AHL molekule. Analize traI mutanta ukazuju na smanjenu proizvodnju kratkolančanih AHL molekula. Preliminarne rezultate potrebno je potvrditi nastavkom istraživanja radi procjenjivanja značaja mutacija.
Abstract (english)
In this study the role of quorum sensing communication in Rhizobium-Leguminosae symbiosis was examined in the process of nitrogen fixation. In order to understand better the quorum sensing communication with the methods of molecular engineering mutants in cinR and traI genes, which are the part of the bacterial strains genome of Rhizobium leguminosarum bv. viciae UPM791, were constructed. Genes cinR and traI are chosen because they control the AHL-dependent quorum sensing system. Mutants of genes cinI and traI are successfully obtained and further characterized at the laboratory for phenotypes in interactions with plants. Preliminary results obtained by TLC analysis showed that the cinR mutant is producing less C14-HSL signals, also showing a reduction on the level of short-chain AHLs. Analysis of traI gene showed no significant alteration on C14-HSL production but the level of short-chain AHLs is reduced. Preliminary results have to be confirmed in further analysis to assess better examination of the significance of mutations
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:417404
Study programmeTitle: Ecology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2017-04-25 12:53:01

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