prikaz prve stranice dokumenta Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.
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master's thesis
Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.
Zagreb: University of Zagreb, Faculty of Science, 2012. urn:nbn:hr:217:612427
Bartulović, Ana
University of Zagreb
Faculty of Science
Department of Biology

Cite this document

Bartulović, A. (2012). Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp. (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:612427

Bartulović, Ana. "Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:612427

Bartulović, Ana. "Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.." Master's thesis, University of Zagreb, Faculty of Science, 2012. https://urn.nsk.hr/urn:nbn:hr:217:612427

Bartulović, A. (2012). 'Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.', Master's thesis, University of Zagreb, Faculty of Science, accessed 16 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:612427

Bartulović A. Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp. [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2012 [cited 2024 April 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:612427

A. Bartulović, "Mehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:217:612427

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Metadata
TitleMehanizmi rezistencije na cefalosporine treće generacije u bakterija Enterobacter spp.
Title (english)Mechanisms of resistance to third generation cephalosporins in Enterobacter spp
AuthorAna Bartulović
MentorBranka Bedenić (mentor)
MentorIvana Ivančić Baće (mentor)
Committee memberIvana Ivančić Baće (predsjednik povjerenstva)
Committee memberBranka Bedenić (član povjerenstva)
Committee memberDubravka Hranilović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2012-02-16, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Abstract
Sojevi bakterija vrste Enterobacter spp. su važni uzročnici bolničkih infekcija. Otpornost na antibiotike, posebice na cefalosporine treće generacije, je stalno u porastu. Otpornost može nastati zbog produkcije inducibilnih β-laktamaza, dereprimiranja kromosomskih β-laktamaza, uslijed produkcije β-laktamaza proširenog spektra (ESBL) ili zbog kombinacije navedenih mehanizama. U ovom diplomskom radu su se razjasnili mehanizmi otpornosti prikupljenih izolata na ispitivane antibiotike. Disk-difuzijskom metodom dokazan je porast otpornosti bakterija na cefalosporine treće generacije, što je potvrđeno metodom MIK. Cefoksitin-cefotaksim antagonističkim testom pokazano je da su svi izolati bili negativni na inducibilnost kromosomske AmpC β-laktamaze. Dvostrukim disksinergističkim testom pokazano je da su svi izolati bili ESBL pozitivni, što je potvrđeno CLSI testom. Svi izolati su bili pozitivni na hiperprodukciju kromosomske AmpC β- laktamaze, što je pokazano testom s bornom kiselinom. Modificiranim Hodge-ovim testom nađena su 2 soja producenta karbapenemaza. Metodom PCR ustanovljeno je da je 43% proučavanih sojeva proizvodilo β-laktamazu TEM, a 60% sojeva proizvodilo β- laktamazu CTX-M.
Abstract (english)
Strains of Enterobacter spp. are important causes of nosocomial infections. Resistance to antibiotics, especially to third generation cephalosporins, is constantly increasing. Resistance can be mediated through production of inducible β-lactamases, derepressed chromosomal β-lactamases, extended spectrum β-lactamases (ESBL) or through combination of these mechanisms. The purpose of this research was to clarify the resistance mechanisms to antibiotics in question. Disk-diffusion method showed the rise of bacterial resistance to third generation cephalosporins, which was confirmed by MIC method. Cephoxitinecephotaxime antagonistic test showed that none of the isolates produced inducible chromosomal AmpC β-lactamases. Double disk-synergy test showed that all the isolates were ESBL positive, which was confirmed by CLSI test. All the isolates showed chromosomal AmpC β-lactamase hyperproduction, which was detected by boronic acid test. Modified Hodge test showed that two bacterial strains were producing carbapenemases. PCR method showed that 43% of the isolates was positive to TEM β- lactamase production and 60% of the isolates was positive to CTX-M β-lactamase production.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:612427
Study programmeTitle: Molecular Biology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2017-04-28 09:33:05

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