Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica

Jančin, Jana

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master's thesis

Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica

Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:524410

Jančin, Jana

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Jančin, J. (2022). Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:524410

MLA 8th Edition

Jančin, Jana. "Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:524410

Chicago 17th Edition

Harvard

Jančin, J. (2022). 'Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 21 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:524410

Vancouver

Jančin J. Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2025 January 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:524410

IEEE

J. Jančin, "Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:524410

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:524410

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Title	Molekularne metode prikladne za praćenje kimerizma nakon transplantacije krvotvornih matičnih stanica
Title (english)	Molecular methods of choice for chimerism monitoring after hematopoietic stem cell transplantation
Author	Jana Jančin
Mentor	Zorana Grubić (mentor)
Committee member	Zorana Grubić (predsjednik povjerenstva)
Committee member	Ana Galov (član povjerenstva)
Committee member	Petra Korać (član povjerenstva)
Committee member	Nada Oršolić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2022-09-27, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Uspjeh transplantacije krvotvornih matičnih stanica (TKM) procjenjuje se analizom statusa kimerizma odnosno, udjela primateljevih stanica u uzorku bolesnika nakon TKMS. Cilj istraživanja bio je usporediti dvije molekularne metode u praćenju kimerizma: metodu umnažanja kratkih uzastopnih ponavljanja DNA lančanom reakcijom (PCR-STR) i metodu lančane reakcije polimerazom u stvarnom vremenu (qPCR) u svrhu određivanja pouzdanosti, osjetljivosti i preciznosti metoda ovisno o omjeru primateljevih i davateljevih stanica u uzorku bolesnika nakon TKMS. Istraživanje je obuhvatilo 12 parova primatelj-davatelj te sveukupno 42 uzorka kimera u kojima je udio primateljevih stanica bio u rasponu od 10-90%. Od 21 analiziranog lokusa STR, najinformativnijima su se pokazali lokusi D13S797, D18S976 i D21S1442, dok je među 30 biljega qPCR za daljnu analizu su najčešće upotrebljavani biljezi KMR040 i KMR050. Raspon dobivenih vrijednosti za udio primateljevih stanica su u prosjeku bio je manji za lokuse STR nego raspon dobiven analizom biljega qPCR. Istraživanje je pokazalo da je metoda qPCR manje pouzdana od metode PCR-STR u određivanju udjela primateljevih stanica kod bolesnika s >10% udjela vlastitih stanica u uzorku nakon TKMS. Zaključno, metoda PCR-STR je bolji izbor za praćenje kimerizma kod bolesnika s visokim udjelom primateljevih stanica.
Abstract (english)	Success of hematopoietic stem cell transplantation (HSCT) is evaluated by chimerism analysis, which is based on determination of recipient and donor cells ratio in patient's sample after HSCT. Purpose of this study was to compare two molecular methods in chimerism monitoring: Short Tandem Repeat-Polymerase Chain Reaction (STR-PCR) and Real time-Polymerase Chain Reaction (qPCR), in order to determine their reliability, sensitivity and accuracy depending on ratio of recipients and donors cells in recipient’s sample after HSCT. This study included 12 recipient-donor pairs and altogether 42 post-HSCT samples in which recipient’s cell ratio was in the range of 10-90%. Out of 12 analysed STR loci the most informative ones were D13S797, D18S976 and D21S1442, while among 30 investigated qPCR markers, KMR040 and KMR050 were most frequently used for chimerism analysis. Range of values obtained for percentage of recipient cells was lower for STR loci in comparison to the one obtained by analysis of qPCR markers. The study showed that qPCR is less reliable then STR-PCR for chimerism monitoring for patients with >10% of their own cells in post HSCT sample. In conclusion, PCR-STR method is a better choice for chimerism status monitoring of those patients with a higher percentage of recipient cells.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:524410
Study programme	Title: Experimental Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access Embargo expiration date: 2023-09-27
Terms of use
Repository	Repository of the Faculty of Science
Created on	2022-10-21 10:18:54