Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina

Knechtl, Patricija

prikaz prve stranice dokumenta Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina

master's thesis

Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina

Zagreb: University of Zagreb, Faculty of Science, 2022. urn:nbn:hr:217:188517

Knechtl, Patricija

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Knechtl, P. (2022). Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:188517

MLA 8th Edition

Knechtl, Patricija. "Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina." Master's thesis, University of Zagreb, Faculty of Science, 2022. https://urn.nsk.hr/urn:nbn:hr:217:188517

Chicago 17th Edition

Harvard

Knechtl, P. (2022). 'Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina', Master's thesis, University of Zagreb, Faculty of Science, accessed 16 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:188517

Vancouver

Knechtl P. Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2022 [cited 2025 January 16] Available at: https://urn.nsk.hr/urn:nbn:hr:217:188517

IEEE

P. Knechtl, "Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:217:188517

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:188517

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Title	Utjecaj brzine rasta na efikasnost adaptacije sustavom CRISPR-Cas u bakterija Escherichia coli različitih genetičkih pozadina
Title (english)	The influence of growth rate on the efficiency of the CRISPR-Cas mediated adaptation in Escherichia coli of different genetic backgrounds
Author	Patricija Knechtl
Mentor	Ivana Ivančić Baće (mentor)
Mentor	Damjan Mitić (komentor)
Committee member	Ivana Ivančić Baće (predsjednik povjerenstva)
Committee member	Mirela Sertić Perić (član povjerenstva)
Committee member	Draginja Mrvoš-Sermek (član povjerenstva)
Committee member	Željka Soldin (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2022-10-25, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Sustav CRISPR-Cas sastoji se od lokusa CRISPR koji zajedno s pridruženim genima cas omogućuje adaptivnu imunost mnogim bakterijama i većini arheja protiv stranih genetičkih elemenata. Lokus CRISPR sastoji se od brojnih ponavljajućih sekvenci DNA koje su razdvojene kratkim razmaknicama strane DNA. Naivna adaptacija prva je faza zaštite u kojoj se kratki fragmenti iz stranih organizama ugrađuju u lokus CRISPR kako bi se zaštitila stanica od budućih napada istom stranom DNA. U ovom radu istražen je utjecaj ekspresije gena cas1 i cas2 na brzinu rasta stanica, određena je faza rasta u kojoj dolazi do ugradnje nove razmaknice te je istražena efikasnost adaptacije u tri različita bakterijska soja E. coli koji imaju tri različite genetičke pozadine (BW25113, AB1157 i MG1655). Također, istraženo je kako količine proteina Cas1 i Cas2 utječu na efikasnost naivne adaptacije (ugradnju razmaknice). Na temelju analize dobivenih rezultata može se zaključiti kako se usporeni rast bakterijskih stanica javlja zbog ugradnje razmaknice u lokus CRISPR, u ranoj log fazi rasta, samo u genetičkoj pozadini BW25113. U bakterijskim sojevima koji su rasli sporo došlo je do ekspresije proteina Cas1 i Cas2, dok geni rpoS i crl nemaju ulogu u izgradnji adaptivne imunosti procesom naivne adaptacije.
Abstract (english)	The CRISPR-Cas system consists of the CRISPR locus, which, together with the CRISPRassociated (cas) genes, provides adaptive immunity to many bacteria and most archaea against foreign genetic elements. The CRISPR locus consists of numerous repetitive DNA sequences separated by short spacers that are derived from foreign DNA. Naïve adaptation is the first phase of protection in which short fragments from foreign organisms are incorporated into the CRISPR locus to protect the cell from future attacks by the same foreign DNA. In this research, we investigated the influence of cas1 and cas2 gene expression on cell growth rate, we determined in which growth phase a new spacer is incorporated and we investigated the efficiency of adaptation in three different bacterial strains of E. coli with three different genetic backgrounds (BW25113, AB1157 and MG1655). Also, we investigated how the amounts of Cas1 and Cas2 proteins affect the efficiency of adaptation (spacer acquisition). Based on the analysis of the obtained results, it can be concluded that the slowed growth of bacterial cells occurs due to the insertion of a spacer into the CRISPR locus in the early log phase of growth only in the BW25113 genetic background. In bacterial strains that grew slowly, Cas1 and Cas2 proteins were expressed. Genes rpoS and crl have no role in building CRISPR immunity through the process of naïve adaptation.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:188517
Study programme	Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access Embargo expiration date: 2023-08-25
Terms of use
Repository	Repository of the Faculty of Science
Created on	2022-11-03 13:08:47