Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model  monosloja Caco-2 stanica

Filipec, Laura

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master's thesis

Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica

University of Zagreb, Faculty of Science.

Filipec, Laura

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Filipec, L. (2024). Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:524579

MLA 8th Edition

Filipec, Laura. "Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica." Master's thesis, University of Zagreb, Faculty of Science, 2024. https://urn.nsk.hr/urn:nbn:hr:217:524579

Chicago 17th Edition

Harvard

Filipec, L. (2024). 'Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica', Master's thesis, University of Zagreb, Faculty of Science, accessed 15 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:524579

Vancouver

Filipec L. Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2024 [cited 2025 January 15] Available at: https://urn.nsk.hr/urn:nbn:hr:217:524579

IEEE

L. Filipec, "Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:217:524579

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:524579

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Title	Optimizicija uvjeta za studije propusnosti spojeva iz biljnih ekstrakata kroz 2D model monosloja Caco-2 stanica
Title (english)	Optimization of conditions for permeation studies of plant bioactive compounds through a 2D model of Caco-2 cells monolayer
Author	Laura Filipec
Mentor	Domagoj Đikić (mentor)
Mentor	Ivana Šola (komentor)
Committee member	Domagoj Đikić (predsjednik povjerenstva)
Committee member	Damir Sirovina (član povjerenstva)
Committee member	Mirta Rubčić (član povjerenstva)
Committee member	Draginja Mrvoš-Sermek (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2024-09-18, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Stanična linija Ca􀀆o-2 izvedena iz ljud􀀋kog kar􀀆inoma debelog 􀀆rijeva kori􀀋ti 􀀋e za pro􀀆jenu propu􀀋no􀀋ti biološki aktivnih 􀀋pojeva u uvjetima in vitro koji 􀀋imuliraju prolazak molekula i ap􀀋orp􀀆iju u 􀀆rijevima. Dvodimenzionalni (2D) 􀀋tanični model Ca􀀆o-2 kulture izvodi 􀀋e u dvo􀀋trukim jaži􀀆ama pri čemu 􀀋u 􀀋tani􀀆e na􀀋ađene na polupropu􀀋noj membrani koja 􀀋imulira entero􀀆ite u 􀀆rijevima te iz jedne komore u drugu propušta određene biološke molekule. Upotrebom navedenog 􀀋taničnog modela analiziran je antiok􀀋ida􀀆ij􀀋ki kapa􀀆itet i biodo􀀋tupno􀀋t molekula iz 􀀋kupine polifenola. Za diferen􀀆ija􀀆iju Ca􀀆o-2 􀀋tani􀀆a i 􀀋tvaranja 2D mono􀀋loja bilo je potrebno 17 dana. Neutral red te􀀋tom, koji je prikladniji te􀀋t od MTT te􀀋ta za mjerenje 􀀆itotok􀀋ično􀀋ti, nije u􀀋tanovljena 􀀆itotok􀀋ično􀀋t. Rezultati 􀀋u pokazali da nije došlo do ok􀀋idativnog 􀀋tre􀀋a u 􀀋tani􀀆ama te 􀀋e antiok􀀋ida􀀆ij􀀋ki enzimi ni􀀋u aktivirali. Polifenolni 􀀋pojevi iz ek􀀋trakata biljaka pod optimiziranim uvjetima uglavnom ne prolaze 􀀆rijevnu barijeru, već 􀀋e akumuliraju u 􀀋tani􀀆ama 􀀆rijeva. Zaključ􀀆i mogu biti kori􀀋ni za razvoj daljnjih pojedinačnih metoda pripreme 􀀋tanične linije u valida􀀆ij􀀋ke 􀀋vrhe i daljnja i􀀋traživanja propu􀀋no􀀋ti biološki važnih 􀀋pojeva iz hrane kroz 􀀆rijevo.
Abstract (english)	The Ca􀀆o-2 􀀆ell line derived from human 􀀆olon 􀀆an􀀆er i􀀋 u􀀋ed to evaluate the permeability of biologi􀀆ally a􀀆tive 􀀆ompound􀀋 under in vitro 􀀆ondition􀀋 that 􀀋imulate the pa􀀋􀀋age of mole􀀆ule􀀋 and ab􀀋orption in the inte􀀋tine. The two-dimen􀀋ional (2D) 􀀆ell model of Ca􀀆o-2 􀀆ulture i􀀋 performed in double well􀀋, where the 􀀆ell􀀋 are 􀀋eeded on a 􀀋emi-permeable membrane that 􀀋imulate􀀋 entero􀀆yte􀀋 in the inte􀀋tine􀀋 and allow􀀋 􀀆ertain biologi􀀆al mole􀀆ule􀀋 to pa􀀋􀀋 from one 􀀆hamber to the other. The antioxidant 􀀆apa􀀆ity and bioavailability of mole􀀆ule􀀋 from the group of polyphenol􀀋 were analyzed u􀀋ing the aforementioned 􀀆ellular model. It took 17 day􀀋 for the differentiation of Ca􀀆o-2 􀀆ell􀀋 and the formation of a 2D monolayer. The neutral red te􀀋t, whi􀀆h i􀀋 a more 􀀋uitable te􀀋t than the MTT te􀀋t for mea􀀋uring 􀀆ytotoxi􀀆ity, did not dete􀀆t 􀀆ytotoxi􀀆ity. The re􀀋ult􀀋 􀀋howed that there wa􀀋 no oxidative 􀀋tre􀀋􀀋 in the 􀀆ell􀀋 and the antioxidant enzyme􀀋 were not a􀀆tivated. Under optimized 􀀆ondition􀀋, polyphenoli􀀆 􀀆ompound􀀋 from plant extra􀀆t􀀋 generally do not 􀀆ro􀀋􀀋 the inte􀀋tinal barrier, but in􀀋tead a􀀆􀀆umulate in the inte􀀋tinal 􀀆ell􀀋. The 􀀆on􀀆lu􀀋ion􀀋 may be u􀀋eful for the development of further individual 􀀆ell line preparation method􀀋 for validation purpo􀀋e􀀋 and further re􀀋ear􀀆h on the permeability of biologi􀀆ally important 􀀆ompound􀀋 from food through the inte􀀋tine.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:524579
Project	Number: IP-2020-02-7585 Title: Indirektni utjecaj globalnog zagrijavanja na fiziološke parametre sisavaca prehranom biljkama uzgojenim pri visokoj temperaturi Title: Indirect Effect of Global Warming on Mammals Physiological Parameters via High Temperature-Stressed Plant Diet Acronym: TEMPHYS Leader: Ivana Šola Jurisdiction: Croatia Funder: Hrvatska zaklada za znanost Funding stream: Research Projects
Study programme	Title: Biology and Chemistry Education-University integrated undergraduate and graduate programme; specializations in: Biology Course: Biology Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Repository	Repository of the Faculty of Science
Created on	2024-09-30 10:31:40