Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.)

Mohorović, Petar

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master's thesis

Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.)

Zagreb: University of Zagreb, Faculty of Science, 2017. urn:nbn:hr:217:216748

Mohorović, Petar

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Mohorović, P. (2017). Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:216748

MLA 8th Edition

Mohorović, Petar. "Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.)." Master's thesis, University of Zagreb, Faculty of Science, 2017. https://urn.nsk.hr/urn:nbn:hr:217:216748

Chicago 17th Edition

Harvard

Mohorović, P. (2017). 'Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.)', Master's thesis, University of Zagreb, Faculty of Science, accessed 18 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:216748

Vancouver

Mohorović P. Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2017 [cited 2024 April 18] Available at: https://urn.nsk.hr/urn:nbn:hr:217:216748

IEEE

P. Mohorović, "Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:217:216748

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:216748

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Title	Učinak promijenjene ekspresije gena BPM na de novo metilaciju DNA uročnjaka (Arabidopsis thaliana L.)
Title (english)	Effect of altered BPM gene expression on de novo DNA methylation in Arabidopsis thaliana L.
Author	Petar Mohorović
Mentor	Nataša Bauer (mentor)
Committee member	Nataša Bauer (predsjednik povjerenstva)
Committee member	Željka Vidaković-Cifrek (član povjerenstva)
Committee member	Vesna Benković (član povjerenstva)
Committee member	Dunja Leljak-Levanić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2017-01-30, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Proteini BPM, kojima je dosada opisana uloga u degradaciji proteina putem ubikvitin proteasoma, ulaze u interakciju s proteinima koji su uključeni u de novo metilaciju DNA uročnjaka Arabidopsis thaliana. Nije poznato utječu li proteini BPM na metiliranost molekule DNA, a kako bi se provjerila potencijalna uloga proteina BPM u metilaciji DNA, koristile su se linije biljaka u kojima je promijenjena ekspresija gena BPM. Analizirala se metiliranost lokusa molekule DNA koji bi mogli biti pod utjecajem de novo metilacije. Analiza metiliranosti DNA provela se enzimatskom digestijom restrikcijskim enzimima koji su ovisni ili osjetljivi na metilaciju DNA, nakon koje je uslijedilo umnažanje istraživanih lokusa lančanom reakcijom polimerazom. Nakon gel elektroforeze i denzitometrijske obrade, te usporedbe rezultata dobivenih za nepocijepanu DNA i DNA pocijepanu metilacijski ovisnim enzimima MspJI i metilacijski osjetljivim enzimima HaeIII ili AluI, utvrdile su se promjene u metiliranosti u biljaka divljeg tipa kao i u biljaka koje različito eksprimiraju gene BPM. Rezultati pokazuju da promijenjene ekspresije gena BPM dovode do promjene u stupnju metiliranosti ponavljajućih sekvenci molekule DNA dok rezultati istraživanja promotorskih regija određenih gena ne daju jasnu sliku promjene obrasca metiliranosti te su potrebna dodatna istraživanja kako bi se to utvrdilo.
Abstract (english)	BPM proteins, which have a known role in protein degradation via the ubiquitin proteasome pathway, interact with proteins involved in de novo DNA methylation in Arabidopsis thaliana. It is still unknown how BPMs effect DNA methylation. To assess the role of BPMs in DNA methylation, different plant mutants with altered expression of BPM genes were used. DNA methylation analysis was performed using the methylation dependent and methylation sensitive restriction enzymes after which the polymerase chain reaction was used to amplify specific, targeted regions of DNA which may be under the influence of de novo methylation. After the gel electrophoresis and densitometric analysis of the amplified undigested DNA and DNA digested with methylation dependant MspJI enzyme and with methylation sensitive HaeIII or AluI enzymes, changes of DNA methylation in wild type, as well in plants with altered BPM gene expression,were determined. Results show that there is a change in methylation pattern in repeating DNA sequences while the results of examined promotor regions of specific genes don't give clear image on how the methylation pattern changes so further research is needed.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:216748
Study programme	Title: Molecular Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-05-22 09:37:30

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