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master's thesis
Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi
Zagreb: University of Zagreb, Faculty of Science, 2018. urn:nbn:hr:217:287186
Pereža, Lora
University of Zagreb
Faculty of Science
Department of Chemistry

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Pereža, L. (2018). Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:287186

Pereža, Lora. "Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:287186

Pereža, Lora. "Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi." Master's thesis, University of Zagreb, Faculty of Science, 2018. https://urn.nsk.hr/urn:nbn:hr:217:287186

Pereža, L. (2018). 'Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi', Master's thesis, University of Zagreb, Faculty of Science, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:287186

Pereža L. Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2018 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:217:287186

L. Pereža, "Elektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:217:287186

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Metadata
TitleElektrokemijska analiza polisaharida kitozana i proteina BSA u morskoj vodi
Title (english)Electrochemical analysis of polysaccharide chitosan and BSA protein in seawater
AuthorLora Pereža
MentorSlađana Strmečki Kos (mentor)
Committee memberNives Galić (predsjednik povjerenstva)
Committee memberŽeljka Soldin (član povjerenstva)
Committee memberMorana Dulić (član povjerenstva)
Committee memberSnežana Miljanić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Chemistry)
Zagreb
Defense date and country2018-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Chemistry
Abstract
U radu su prvi put istraženi detalji elektrokemijske analize modelnog aminopolisaharida kitozana, proteina goveđeg serum albumina (BSA), te njihove smjese u morskoj vodi. Mjerenja su izvedena na visećoj živinoj kapi koristeći adsorptivnu kronopotenciometriju s pobudnom strujom (CPS). Oba analita dala su karakterističan pik H (Ep = ~ –1.8 V) kao posljedicu katalitičke redukcije vodikovih iona. Iako je samo BSA bilo moguće detektirati in situ u morskoj vodi, za oba analita je najosjetljivija detekcija bila nakon adsorptivnog prijenosa (AdT) iz morske vode u fosfatni pufer pH ~ 7,0. Stoga su definirani optimalni eksperimentalni uvjeti za njihovu AdT CPS analizu: potencijal akumulacije, Ea= –0,1 V, vrijeme akumulacije, ta = 60 s, te pobudna struja, Istr = –20 μA za kitozan te Istr = –40 μA za BSA. AdT CPS detekcijom smjese dobiven je samo jedan pik H čime selektivna detekcija kitozana, a ni BSA u njihovoj smjesi nije bila moguća.
Abstract (english)
The thesis explored for the first time the details of electrochemical analysis of chitosan aminopolysaccharide, BSA protein, and their seawater mixture. Measurements were performed on a hanging mercury drop electrode by using constant current chronopotentiometric stripping (CPS). Both analytes gave a characteristic peak H (Ep = ~ –1.8 V) due to catalytic hydrogen evolution reaction. Although only BSA can be detected in situ in seawater, the most sensitive detection for both analytes was after an adsorptive transfer (AdT) from seawater to phosphate buffer pH ~ 7.0. Therefore, optimal experimental conditions for their AdT CPS analysis were defined: accumulation potential, Ea = –0.1 V, accumulation time, ta = 60 s, and stripping current, Istr = –20 μA for chitosan and Istr = –40 μA for BSA. Only one AdT CPS peak H was observed in mixture, so selective detection of neither chitosan nor BSA in their mixture was possible.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:217:287186
Study programmeTitle: Graduate university studies in chemistry; specializations in: Research programme
Course: Research programme
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2020-09-28
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Science
Created on2018-11-22 12:21:18

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