Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG

Kovačević, Jelena

prikaz prve stranice dokumenta Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG

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master's thesis

Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG

Zagreb: University of Zagreb, Faculty of Science, 2013. urn:nbn:hr:217:496612

Kovačević, Jelena

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Kovačević, J. (2013). Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:496612

MLA 8th Edition

Kovačević, Jelena. "Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG." Master's thesis, University of Zagreb, Faculty of Science, 2013. https://urn.nsk.hr/urn:nbn:hr:217:496612

Chicago 17th Edition

Harvard

Kovačević, J. (2013). 'Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG', Master's thesis, University of Zagreb, Faculty of Science, accessed 19 April 2024, https://urn.nsk.hr/urn:nbn:hr:217:496612

Vancouver

Kovačević J. Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2013 [cited 2024 April 19] Available at: https://urn.nsk.hr/urn:nbn:hr:217:496612

IEEE

J. Kovačević, "Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:217:496612

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Title	Transgenične biljke Arabidopsis thaliana (Heynh.) koje eksprimiraju protein TROL obilježen proteinskim biljezima HA i FLAG
Title (english)	Generation of Arabidopsis thaliana (Heynh.) plants containing the TROL protein labeled with HA and FLAG protein markers
Author	Jelena Kovačević
Mentor	Hrvoje Fulgosi (mentor)
Committee member	Nataša Bauer (predsjednik povjerenstva)
Committee member	Hrvoje Fulgosi (član povjerenstva)
Committee member	Željka Vidaković-Cifrek (član povjerenstva)
Committee member	Dijana Škorić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2013-02-05, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Transformacija biljaka metodom “floral dip” postupak je unosa T-DNA sekvence u genom stanica ovarija prije oplodnje. U ovom radu opisala sam transformaciju biljaka Arabidopsis thaliana molekulom T-DNA iz plazmidnog vektora pH7WG2,0 TROL-HA-FLAG. TROL je transmembranski protein koji sudjeluje u regulaciji puta prijenosa elektrona na tilakoidnim membranama kloroplasta. Dodatak proteinskih biljega HA i FLAG na protein TROL olakšava njegovu imunodetekcijsku provjeru i omogućuje daljnja istraživanja funkcije proteina TROL. Kako bih dokazala uspješnost transformacije biljaka genom za protein TROL-HA-FLAG, biljke sam analizirala metodama selekcije sjemenki na hranjivoj podlozi s antibiotikom, PCR provjere genoma i imunodetekcije proteina u listovima odraslih biljaka. U transformiranim biljkama sam imunodetekcijom potvrdila lokalizaciju proteina TROL-HA-FLAG u tilakoidnim membranama kloroplasta. Daljnja istraživanja omogućit će detaljniju potvrdu funkcionalnosti proteina TROL-HA-FLAG.
Abstract (english)	Floral dip is a transformation method that results in the insertion of T-DNA into the genome of unfertilized ovary cells. This thesis describes the transformation of Arabidopsis thaliana plants with T-DNA from pH7WG2,0 TROL-HA-FLAG plasmid vector. TROL is a transmembrane protein that regulates electron transport flow on the thylakoid membranes of chloroplasts. Addition of protein tags HA and FLAG to the TROL protein allows easier immunodetection analysis and enables further studies of the plant TROL protein function. In order to show the success of plant transformation with the TROL-HA-FLAG protein gene, the plants were subjected to multiple analyses such as seed selection method on growth plates with an added antibiotic, plant DNA PCR analysis and immunodetection analysis of plant leaf proteins. Due to the immunodetection analysis, I managed to confirm the localization of TROL-HA-FLAG protein in chloroplast thylakoid membranes of transformed plants. Further research could better show if TROL-HA-FLAG protein function is diminished.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:496612
Study programme	Title: Molecular Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Science
Created on	2017-04-27 09:26:49

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