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master's thesis
Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor
Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2010. urn:nbn:hr:109:805910
Dropulja, Ivana
Josip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
Department of Process Engineering
Sub-department of Thermodynamics and Reaction Engineering

Cite this document

Dropulja, I. (2010). Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY. Retrieved from https://urn.nsk.hr/urn:nbn:hr:109:805910

Dropulja, Ivana. "Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2010. https://urn.nsk.hr/urn:nbn:hr:109:805910

Dropulja, Ivana. "Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor." Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, 2010. https://urn.nsk.hr/urn:nbn:hr:109:805910

Dropulja, I. (2010). 'Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor', Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, accessed 25 April 2024, https://urn.nsk.hr/urn:nbn:hr:109:805910

Dropulja I. Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY; 2010 [cited 2024 April 25] Available at: https://urn.nsk.hr/urn:nbn:hr:109:805910

I. Dropulja, "Oksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor", Master's thesis, Josip Juraj Strossmayer University of Osijek, FACULTY OF FOOD TECHNOLOGY, Osijek, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:109:805910

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Metadata
TitleOksidacija fenolnih spojeva katalizirana vlastito proizvedenom lakazom iz Trametes versicolor
Title (english)Oxidation of phenolic compounds catalyzed by personally produced laccase from Trametes versicolor
AuthorIvana Dropulja
MentorĐurđa Vasić-Rački (mentor)
Committee memberDamir Magdić (član povjerenstva)
Committee memberĐurđa Vasić-Rački (član povjerenstva)
Committee memberIvica Strelec (član povjerenstva)
Committee memberVinko Krstanović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
FACULTY OF FOOD TECHNOLOGY
(Department of Process Engineering)
(Sub-department of Thermodynamics and Reaction Engineering)
Osijek
Defense date and country2010-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Engineering
Abstract
Gljiva bijelog truljenja Trametes versicolor proizvodi tri lignolitička enzima: lakazu, lignin peroksidazu i mangan peroksidazu te posjeduje sposobnost razgradnje lignina, policikličkih aromatskih ugljikovodika i ostalih organskih sintetskih spojeva. Lakaze (EC 1.10.3.2) su oksidaze koje kataliziraju oksidaciju supstrata prijenosom elektrona i istovremenom redukcijom kisika u vodu. Cilj ovog rada bio je proizvodnja lakaze iz Trametes versicolor G-99 u submerznim uvjetima na otpadu iz industrije papira, te oksidacija fenolnih spojeva: fenola, katehola i koniferilnog alkohola, katalizirana vlastito proizvedenom lakazom. Fenolni spojevi su široko rasprostranjeni spojevi u prirodi. Sastavni su dio industrijskih otpadnih voda kao npr. fenol i katehol. S druge strane, mogu biti dio stanične stijenke biljaka kao koniferilni alkohol. Pokusima enzimske oksidacije u kotlastom reaktoru ostvarene su 100%-tne konverzije fenola, katehola i koniferilnog alkohola. Razvijeni matematički model procesa dobro opisuje eksperimentalne podatke.
Abstract (english)
Trametes versicolor is a white-rot fungus that produces three ligninolytic enzymes: laccase, lignin-peroxidase and manganese-peroxidase, and has an efficient degradation capacity of lignin, polycyclic aromatic hydrocarbons and a number of synthetic dyes. Laccases (EC 1.10.3.2) are multi-copper oxidases that catalyze oxidation of substrates by electron transfer and simultaneous reduction of oxygen to water. In this work, Trametes versicolor MZKI G-99 was grown in a submerged culture using waste from paper industry with the aim of laccase production. Once produced, laccase was kinetically characterized and used as a catalyst in the oxidation of three different phenolic compounds: phenol, catechol and coniferyl alcohol, without any purification and concentration steps. Phenolic compounds can be found in numerous industrial wastewaters (phenol and catechol). They can also be the main constituents of the plant cell walls (coniferyl alcohol). The experiments were carried out in a batch reactor and 100% conversion of laccase-catalyzed oxidation of phenol, catechol and coniferyl alcohol was obtained. Mathematical model of the process was developed and fits the experimental data well.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:109:805910
ProjectNumber: 125-1252086-2793
Title: Biokatalizatori i biotransformacije
Title: Biotransformations and biocatalysts
Leader: Đurđa Vasić-Rački
Jurisdiction: Croatia
Funder: MZOS
Funding stream: ZP
Study programmeTitle: Process Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka procesnog inženjerstva (magistar/magistra inženjer/inženjerka procesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology Osijek
Created on2015-12-15 13:11:12

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