MOBA is short for Massive Online Battle Arena and it is one of the most popular types of video games in the 21st century. MOBAs are online games in which ten players, divided into two teams strategically try to take down the main construction of the opposing team. Every player picks one specific role out of six of them in the game. There are two negative stereotypes about female players - female players are worse in MOBA games than male players and female players should only play the role of Support. Research shows that people with a feminine gender role are dominantly socially motivated to play, while people with a masculine gender role are more motivated by power and achievement. The aim of this study was to determine the relation of gender roles and motivation for playing as factors of identification with characters from MOBA games. The study involved 200 participants who completed an online questionnaire. The questionnaire consisted of a questionnaire regarding general information, Bem Sex Role Inventory and Online Gaming Motivations Scale. The results show that people with a different preference of roles in MOBA games, differ in the expression of masculinity but show no significant difference in the expression of femininity. By conducting post-hoc tests no difference was found in power and achievement amongst people who prefer different roles in MOBA games. Also, no significant difference was found in identification and social motivation amongst people who prefer different roles in MOBA games. Moreover, people with different gender roles differ in power and achievement, but also in identification and social motivation for playing MOBA games. Finally, a positive correlation has been found between the expression of masculinity and power/achievement. Also, there was statistically significant positive correlation between the expression of femininity and identification/ social motivation.