Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši

Čelebić, Inga

prikaz prve stranice dokumenta Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši

master's thesis

Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši

Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2021. urn:nbn:hr:178:669266

Čelebić, Inga

University of Zagreb
Faculty of Veterinary Medicine

Cite this document

APA 6th Edition

Čelebić, I. (2021). Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:669266

MLA 8th Edition

Čelebić, Inga. "Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:178:669266

Chicago 17th Edition

Harvard

Čelebić, I. (2021). 'Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 15 September 2024, https://urn.nsk.hr/urn:nbn:hr:178:669266

Vancouver

Čelebić I. Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2021 [cited 2024 September 15] Available at: https://urn.nsk.hr/urn:nbn:hr:178:669266

IEEE

I. Čelebić, "Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:178:669266

Cite this item: https://urn.nsk.hr/urn:nbn:hr:178:669266

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Title	Praćenje titra specifičnih protutijela potaknutog autogenim cjepivom protiv APEC sojeva E. coli na roditeljskim farmama teške pasmine kokoši
Title (english)	Monitoring of specific antibody titers induced by autogenous vaccine against APEC strains of E. coli in broiler parent flocks
Author	Inga Čelebić
Mentor	Željko Gottstein (mentor)
Committee member	Danijela Horvatek Tomić (predsjednik povjerenstva)
Committee member	Željko Gottstein (član povjerenstva)
Granter	University of Zagreb Faculty of Veterinary Medicine Zagreb
Defense date and country	2021-11, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Veterinary Medicine Veterinary Public Health and Food Safety
Abstract	APEC strains (Avian pathogenic Escherichia coli) are the main cause of collibacilosis in poultry. They inflict major economic damage by causing high morbidity and mortality in chicks, broilers and laying hens. Also, chicken meat products contaminated with APEC present danger for human health. Appearance of resistant and multiresistant strains as a result of long term, excessive use of antibiotics, has widely limited attainability of effective antimicrobial substances and forced us to search for more effective options for reducing the incidence of collibacilosis and large economical loses caused by it. Production and administration of autogenous vaccines is a very effective method in disease prevention. In this research, microbiological monitoring of the delivered carcasses enabled isolation of E. coli strains in the broiler parent flock farms. After that, typing of the isolates was conducted and an autogenous vaccine was produced for vaccination of pullets and roosters at 9 and 19 weeks of age. For determining specific antibody titre, an ELISA test was made from inactivated antigen used for manufacturing of the vaccine. Results proved a significant titre increase after application of the autogenous vaccine. Application of aluminium hydroxide as adjuvant stimulated fast rise of the titre, but also a constant drop of titre. Oil adjuvant on the other hand stimulates long lasting titre which is double in values in vaccinated flocks compared to unvaccinated older flocks.
Abstract (english)	APEC strains (Avian pathogenic Escherichia coli) are the main cause of collibacilosis in poultry. They inflict major economic damage by causing high morbidity and mortality in chicks, broilers and laying hens. Also, chicken meat products contaminated with APEC present danger for human health. Appearance of resistant and multiresistant strains as a result of long term, excessive use of antibiotics, has widely limited attainability of effective antimicrobial substances and forced us to search for more effective options for reducing the incidence of collibacilosis and large economical loses caused by it. Production and administration of autogenous vaccines is a very effective method in disease prevention. In this research, microbiological monitoring of the delivered carcasses enabled isolation of E. coli strains in the broiler parent flock farms. After that, typing of the isolates was conducted and an autogenous vaccine was produced for vaccination of pullets and roosters at 9 and 19 weeks of age. For determining specific antibody titre, an ELISA test was made from inactivated antigen used for manufacturing of the vaccine. Results proved a significant titre increase after application of the autogenous vaccine. Application of aluminium hydroxide as adjuvant stimulated fast rise of the titre, but also a constant drop of titre. Oil adjuvant on the other hand stimulates long lasting titre which is double in values in vaccinated flocks compared to unvaccinated older flocks.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:178:669266
Study programme	Title: Veterinary Medicine Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Veterinary Medicine
Created on	2021-12-01 12:39:25