The paper is based on the theory followed by a research carried out by the author, in other words a practical part of the culture of entrepreneurship on the example of the Croatian company DOK - ING d.o.o. It is very important to know the business culture of a particular country because it is one of the most important factors of professional growth and development. The brief general chapters on entrepreneurship, culture and ethical principles are introduced into the core of the work of entrepreneurship in the Republic of Croatia, which is the foundation of the research. Organizational culture is one of the key factors affecting the organization's business and existence, while business culture is part of the general culture from which it derives. These terms, as well as other concepts related to the subject such as entrepreneurship, entrepreneurship, enterprise, corporate entrepreneurship, and more, will be clarified in the work as well as some of the historical facts, business ethics and social responsibility of entrepreneurs. Through the conducted research, the use of business culture is seen on the example in one of Croatias companies, and thus it is noticed what kind of culture is in the area in which the respondents stay on a daily basis. The questionnaire identifies the extent to which respondents are aware of entrepreneurial culture and its importance, and following the careful research of the topic, the interviewing, the questionnaire and the processing of the data, conclusions were made regarding their business.