Caper bush (Capparis orientalis Veill.) is a wild plant species whose habitat is most closely associated with the Mediterranean area where it has spread most natural. This plant species has very low growing requirements. It is resistant to drought and lack of water and develops a very strong and deep root. Due to its significant aromatic and medicinal characteristics, there is a growing interest in commercial caper cultivation. The problem with cultivation is difficult rooting of this species. Today, the method of propagation in in vitro aseptic conditions, the so-called micropropagation which could be a potential solution for the propagation of this and many other plant species. In this way, it is possible to propagate the mother plant under control conditions and on a medium of precisely defined composition adapted to the species being introduced into the culture. In order to improve rooting in this study, caper has been introduced into the culture where it first multiplied on a medium with two different growth regulators. Cytokines, 6-benzylaminopurine (BAP) and meta-topoline (mT) were used in four different concentrations. After achieving the desired number of explants on the multiplication nutrient medium, the caper shoots were placed on the rooting medium with auxin indolyl-3-butyric acid (IBA) and the control medium without the addition of hormones. The aim of the study was to investigate the success of rooting of capers independently of the cytokines used in the shoot multiplication phase. The results showed that there was no significant difference between BAP and mT in the rooting process, while a higher percentage of successfully acclimatized plants was in plants formed on medium with mT.