In intensive agricultural production significant amounts of fertilizers and plant protection products are used to ensure high and stable yields. This research quantified the transport of nitrates (NO3-) on hillslope soil by measuring its concentration in leachates and using a combination of field monitoring and research under controlled conditions. Nitrates in water samples from lysimeters were determined spectrophotometrically using the segmented flow method on an ion analyzer, and nitrate concentrations from water samples under controlled conditions were measured using multiparameter photometer. Leachate pH and electrical conductivity (E.C.) were also measured using pH/EC meter. The highest concentration of NO3‒ was leached from the soil column sampled from the bottom of the hillslope in the row area of the vineyard, and the lowest from the soil column sampled from the top of the hillslope in the interrow area. Statistically significant difference with respect to the position on the hillslope was found for E.C. and NO3‒ concentration, but not for pH. The highest values of E.C. and NO3‒ concentrations were at the bottom of the hillslope. The lowest E.C. was measured at the middle of the hillslope, and the lowest NO3‒ concentration was recorded at the top of the hillslope. Results suggest that the slope of the terrain has a statistically significant effect on the nitrate concentration in the leachate.