Vaccination (immunization, active immunization) is a procedure of introducing an antigen or group of antigens within the host organism in the form of a vaccine and for the purpose of achieving an immune, protective response of the organism and preventing the development of a disease caused by the infectious agent or its products. Vaccination has eliminated many infectious diseases that have killed millions of children and adults in the last century, improved health and the quality of life in general. The aim of this final thesis was to establish and determine the knowledge of undergraduate nursing students at the Faculty of Health Studies in Rijeka on vaccination as well as to explore their views and attitudes on this topic. The study included a total of 65 students. An anonymous survey questionnaire developed in the Google forms program, obtained the results of which a detailed processing in Microsoft Excel, concluded. The obtained data indicate that students have adequate knowledge about vaccination and also have positive attitudes on this topic.A more detailed analysis of the obtained data indicates that the second year has the most knowledge on this topic, while the first year has the least knowledge on this topic, which also confirmed the hypothesis of this final work. Considering that vaccination is a topic of frequent conflicts today, it is important to educate health professionals so that they can transfer their acquired knowledge to the general population and thus ensure the care of the population to preserve their own health and the health of their loved ones.