Physiotherapists are health professionals who participate in the patient's rehabilitation program and who motivate patients to be physically active. The aim of this cross-sectional research was to compare the physical activity and quality of life of physiotherapy students in Rijeka and Seinäjoki. The study involved 147 students (76 from Seinäjoki and 71 from Rijeka). According to the results, all respondents had the same overall physical activity levels that differed in the domains of physical activity at work, transport, household and leisure time. Students in Seinäjoki had higher results in physical activity levels in leisure time, in bicycle transport, in sedentary behaviour but lower Mental Component Summary Measure. Students in Rijeka had higher results in physical activity at work, household and walking, but lower Physical Component Summary Measure. Seinäjoki students reported more pain in the ankle or foot area in the last 12 months, that has prevented them from doing daily activities and pain during the last 7 days in the neck and knee area. It is possible that Croatian students are more active in activities that are free of charge because of lower socioeconomic status compared to students from Finland who are more devoted to physical activity in leisure time. From the results obtained, it is concluded that physiotherapy students in this research are more physically active in comparison to WHO recommendations and as such can be positioned as a role-model to patients to promote the benefits of physical activity.