Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)

Horvatinec, Jelena

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master's thesis

Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)

Zagreb: University of Zagreb, Faculty of Agriculture, 2019. urn:nbn:hr:204:588441

Horvatinec, Jelena

University of Zagreb
Faculty of Agriculture
Fisheries, Beekeeping, Game Management and Special Zoology

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APA 6th Edition

Horvatinec, J. (2019). Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.) (Master's thesis). Zagreb: University of Zagreb, Faculty of Agriculture. Retrieved from https://urn.nsk.hr/urn:nbn:hr:204:588441

MLA 8th Edition

Horvatinec, Jelena. "Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)." Master's thesis, University of Zagreb, Faculty of Agriculture, 2019. https://urn.nsk.hr/urn:nbn:hr:204:588441

Chicago 17th Edition

Harvard

Horvatinec, J. (2019). 'Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)', Master's thesis, University of Zagreb, Faculty of Agriculture, accessed 09 November 2024, https://urn.nsk.hr/urn:nbn:hr:204:588441

Vancouver

Horvatinec J. Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Agriculture; 2019 [cited 2024 November 09] Available at: https://urn.nsk.hr/urn:nbn:hr:204:588441

IEEE

J. Horvatinec, "Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)", Master's thesis, University of Zagreb, Faculty of Agriculture, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:204:588441

Cite this item: https://urn.nsk.hr/urn:nbn:hr:204:588441

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Title	Utjecaj dobi i spola na biokemijske značajke hemolimfe medonosne pčele (Apis mellifera L.)
Title (english)	Influence of age and gender on the biochemical properties of the honey bee hemolymph (Apis mellifera L.)
Author	Jelena Horvatinec
Mentor	Lidija Svečnjak (mentor)
Committee member	Lidija Svečnjak (predsjednik povjerenstva)
Committee member	Dragan Bubalo (član povjerenstva)
Committee member	Marko Vinceković (član povjerenstva)
Granter	University of Zagreb Faculty of Agriculture (Fisheries, Beekeeping, Game Management and Special Zoology) Zagreb
Defense date and country	2019-09-30, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Agronomy
Abstract	Hemolimfa je tekućina otvorenog sustava cirkulacije medonosne pčele (Apis mellifera L.) koja predstavlja funkcionalni ekvivalent krvi i limfe sisavaca. Sloţenog je kemijskog sastava te uz šećere i lipidne komponente, sadrţi preko 300 različitih proteina. Dosadašnjim su istraţivanjima utvrĎene odreĎene razlike u sastavu hemolimfe izmeĎu kasti pčela, različitih razvojnih stadija te dobnih skupina pčela. MeĎutim, detaljna kemijska karakterizacija sastava hemolimfe nije provedena. Stoga je cilj ovog rada bio utvrditi biokemijske značajke i razlike u sastavu hemolimfe pčela u ovisnosti o dobi i spolu temeljem kemijskog fingerprintinga hemolimfe. Istraţivanje je provedeno na 3 pokusne pčelinje zajednice. Ekstrakcijom iz područja prsišta iz svake su pokusne zajednice prikupljeni uzorci hemolimfe pet dobnih skupina radilica (1, 7, 14, 24 i 42 dana; n=338) i trutova (n=8) te su analizirani metodom FTIR-ATR spektroskopije. Rezultati istraţivanja pokazali su kako hemolimfa pčela daje karakterističan IR spektar, a temeljem spektralnih varijacija utvrĎeno je kako se hemolimfa pčela ovisno o dobi i spolu ne razlikuje kvalitativno, već postoje razlike u udjelu pojedinih komponenata sastava.
Abstract (english)	Hemolymph is a liquid of the open circulation system of the honey bee (Apis mellifera L.), which representes a functional equivalent of mammalian blood and lymph. It has a complex chemical composition and contains over 300 different proteins along with sugars and lipid components. Previous studies have revealed certain differences in the hemolymph composition between the honey bee castes, different developmental stages and age groups of bees. However, a detailed chemical characterization of the hemolymph composition has not been performed. Therefore, the aim of this study was to determine the biochemical characteristics and differences in honey bee hemolymph composition depending on the age and gender based on the hemolymph chemical fingerprinting. The study was conducted on 3 experimental honey bee colonies. Hemolymph samples of five age groups of workers (1, 7, 14, 24 and 42 days old; n = 338) and drones (n=8) were collected by thorax extraction from each experimental colony, and analysed by FTIR-ATR spectroscopy. The results showed that honey bee hemolymph provides a characteristic IR spectrum, and based on spectral variations it was determined that the hemolymph of the honey bee does not differ qualitatively depending on age and gender, but there are differences in the proportion of individual compositional components.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:204:588441
Study programme	Title: Plant Sciences Study programme type: university Study level: graduate Academic / professional title: magistar / magistra inženjer/inženjerka biljnih znanosti (magistar / magistra inženjer/inženjerka biljnih znanosti)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository Faculty of Agriculture University of Zagreb
Created on	2019-11-07 13:37:09