Different management practices in agriculture can result in soil structure spoiling, for instance disaggregation of the bare soil by downfalls, oxidation of the organic matter caused by increased aeration due to the soil cultivation, mechanical dispersion due to the soil compaction in humid and size degradation by agricultural tillage. Appearance and pore distribution, especially of macropores have significant impact on water flow and solute transport in unsaturated soil zone by determining transport potential of agrochemicals and pollution leaching through soil. Therefore, it is important to quantify the proportion and structure of pore system in the soil sample. The aim of this thesis was to apply different methods to quantify the proportion and the structure of the porous system in the undisturbed soil sample. This was determined with the application of calcium chloride (CaCl2) as a tracer, preferential flow in the soil was estimated using Brilliant Blue FCF and to reproduce real appearance of the porous system in soil the X-ray computed tomography (CT) was used. Using the above mentioned methods porous system of the undisturbed soil from experimental site Baštica was quantified. Solute dispersion was estimated by adding calcium chloride in the concentration of 100 mg L- 1 and consecutive column elution (leaching). Concentrations of the leached chlorides were determined by the continuous flow analyzer. Dispersion coefficient was determined using HYDRUS-1D software. After the dispersion experiment Brilliant Blue FCF was added to the soil column. Soil column was cut alongside and preferential flow was determined. Percentage of blue staining was processed by ImageJ programme. In order to determine soil porosity and reproduce porous system structure, the soil column was scanned with X-ray computed tomography. Pore percentage was determined using the ImageJ software with the necessary image corrections. In this study, the dispersion coefficient of 8.5 cm2 min-1 was determined with the sample porosity of 44.2 vol %. Applying the Brilliant Blue FCF tracer, staining percentage (preferential flow pathways) value was determined in range between 17.212 % and 33.362 %. Using the 3D imaging of the soil column, soil detoriation at the bottom of the column and changes in structure were identified.