The grapevine is one of the most important agricultural crops in the Republic of Croatia. Grapevine can be infected by a large number of known viruses, and according to literature 86 different grapevine viruses have been discovered. Not all viruses are economically significant, but those considered as important are: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFLV), grapevine leafroll-associated virus – 1, 2 and 3 (GLRaV-1,GLRaV-2, GLRaV-3), grapevine fleck virus (GFkV), Grapevine virus A (GVA) and B (GVB). The aim of this study was to determine the presence and frequency of the economically important viruses ArMV, GFLV, GLRaV-1 and GLRaV-3 in the autochthonous variety Babić collected from its main growing areas. In total 232 samples were collected from 10 different locations. Samples were tested using the immunoenzymatic ELISA method. The presence of all viruses included in survey was confirmed. The most common was GLRaV-3 (97,84%), followed by GFLV (31,47%) and GLRaV-1 (5,17%). Determined ArMV frequency of 3,02% is questionable due to low virus concentration. Mixed infections have also been identified and the most common combination was GFLV + GLRaV-3 (29,31%). Of all the tested samples, only in 4 (1,72%) presence of studied viruses was was not confirmed.