Since the last decades of human pressure on nature is becoming greater, thus the spring flowering species are directly threatened by human activities. Every change of temperature of field, every skip of mowing, rising or falling of the water, every hidromeliorative operation etc. has a directly impact on flora and fauna of the certain area. By its ecological features, the area of Lake Ljubljanica – Odransko polje seems very peculiar community of wet meadows with significant changes of wet and dry soil phase. Field research was carried out on three locations, in spring and autumn 2015 and spring 2016. Seven micro–locations were randomly selected where the spring flora was inventoried and plant species were collected and herboried. The analysis of spring flora within the protected area Ljubljanica – Odransko polje, recorded a total of 51 plant species, of which 10 spring flowering species which makes 5.1% of the recorded species. There was also detected a protected species, Marsilea quadrifolia which is listed in the Red Book of Croatian vascular flora. Following types of spring flowering species were recorded: fritillary (Fritillaria meleagris), Cardamine pratensis, some species of the genus Ranunculus sp., spring snowflake (Leucojum vernum), forest wild ginger (Asarum europaeum) and sweet violets (Viola odorata). Spring flowering species are not numerous in this area and their occurrence and abundance should be better monitored.