master's thesis
SEX-SPECIFIC OXIDATIVE AND ANTIOXIDATIVE STATUS IN THE LIVER OF THE CHRONICALLY AND ACUTELY STRESSED RATS

Ana Vuković (2016)
Sveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
Metadata
TitleSpolno-specifični oksidacijski i antioksidacijski status jetre štakora izloženih akutnom i kroničnom stresu
AuthorAna Vuković
Mentor(s)Elizabeta Has Schon (thesis advisor)
Abstract
Akutni i kronični stres mogu potaknuti proizvodnju reaktivnih kisikovih jedinki koje mogu uzrokovati oksidacijski stres, proces koji se smatra ključnim u razvoju brojnih bolesti, između ostaloga i jetrenih. Cilj ovog istraživanja je bio odrediti utjecaj akutnog i kroničnog stresa na razvoj oksidacijskog stresa i antioksidacijski odgovor jetre kod mužjaka (M), neovarijektomiranih (NE-OVX) i ovarijektomiranih (OVX) ženki. Sedamdeset štakora 16 tjedana starosti bili su podijeljeni u 9 skupina: M, NE-OVX i OVX ženke kao kontrolne skupine; M, NE-OVX i OVX ženke izloženi akutnom stresu; mužjaci NE-OVX i OVX ženke izloženi kroničnom stresu. Kao indikator oksidacijskog oštećenja jetre praćena je razina lipidne peroksidacije koja je određena mjerenjem količine reaktivnih supstanci tiobarbiturne kiseline (TBARS). Antioksidacijski status jetre je istražen mjerenjem aktivnosti katalaze, superoksid-dismutaze, glutation-peroksidaze, glutation S-transferaze i glutation-reduktaze te omjera reduciranog i oksidiranog glutationa (GSH/GSSG). I akutni i kronični stres su uzrokovali povećanje količine TBARS-a kod svih skupina u odnosu na kontrolu. Rezultati pokazuju da izloženost akutnom i kroničnom stresu rezultiraju smanjenom antioksidacijskom obranom jer je u pojedinim skupinama smanjen omjer GSH/GSSG kao i aktivnost antioksidacijskih enzima. Ovarijektomija sama po sebi uzrokuje nastanak oksidacijskog stresa. Iz dobivenih rezultata je vidljivo da akutni i kronični stres uzrokuju oksidacijski stres i smanjenje antioksidacijske zaštite u jetri. Također, antioksidacijski odgovor je i djelomično spolno specifičan, a razina oksidacijskog oštećenja je bila najveća kod OVX skupine.
Keywordsacute stress antioxidative enzymes chronic stress GSH/GSSG ratio lipid peroxidation liver ovariectomy oxidative stress rats
Parallel title (English)SEX-SPECIFIC OXIDATIVE AND ANTIOXIDATIVE STATUS IN THE LIVER OF THE CHRONICALLY AND ACUTELY STRESSED RATS
Committee MembersSandra Ečimović (committee chairperson)
Elizabeta Has Schon (committee member)
Ivna Štolfa Čamagajevac (committee member)
GranterSveučilište Josipa Jurja Strossmayera u Osijeku
Odjel za biologiju
PlaceOsijek
StateCroatia
Scientific field, discipline, subdisciplineNATURAL SCIENCES
Biology
Study programme typeuniversity
Study levelgraduate
Study programmeMaster of Science Graduate University Study Programme in Biology; specializations in: Master of Biology
Study specializationMaster of Biology
Academic title abbreviationmag.biol.
Genremaster's thesis
Language Croatian
Defense date2016-09-09
Parallel abstract (English)
Acute and chronic stress can induce production of reactive oxygen species that can cause oxidative stress, which is recognized to be one of the key pathophysiological mechanism in liver and other diseases development. The aim of this study was to estimate the impact of acute and chronic stress on the oxidative stress development and antioxidative status of the liver in males (M), non-ovariectomized (NE-OVX) and ovariectomized (OVX) female rats. Seventy 16-week-old rats were included in the study and divided into nine groups: M, NE-OVX and OVX females that were exposed to acute stress; M, NE-OVX, and OVX females that were exposed to chronic stress; and M and NE-OVX females as a control groups. As an indicator of liver oxidative damage, lipid peroxidation levels expressed in terms of thiobarbituric acid reactive substances (TBARS) were determined, while liver antioxidative status was determined by catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione S-trasferase activities and oxidized/reduced glutathione (GSH/GSSG) ratio. Both acute and chronic stress significantly increased TBARS content in the liver of all groups relative to control groups. Results showed that acute and chronic stress lowered antioxidant defense capacity, as evaluated by the significant decrease in the GSH/GSSG ratio of some groups and decrease of some antioxidative enzymes activities. Ovariectomy itself resulted in the oxidative stress development. The analyzed data indicate that acute and chronic stress induced oxidative stress and decreased antioxidative capacity in rat liver. The oxidative and antioxidative response to acute and chronic stress were partially sex specific, while the intensity of the oxidative stress was the highest in the OVX females.
Parallel keywords (Croatian)akutni stres antioksidacijski enzimi jetra kronični stres lipidna peroksidacija oksidacijski stres omjer GSH/GSSG ovarijektomija štakori
Resource typetext
Access conditionOpen access
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:181:750574
CommitterVesna Radman Meic