Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)

Mihić, Ivan

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master's thesis

Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)

Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology, 2015. urn:nbn:hr:181:824788

Mihić, Ivan

Josip Juraj Strossmayer University of Osijek
Department of biology

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APA 6th Edition

Mihić, I. (2015). Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae) (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:181:824788

MLA 8th Edition

Mihić, Ivan. "Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)." Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, 2015. https://urn.nsk.hr/urn:nbn:hr:181:824788

Chicago 17th Edition

Harvard

Mihić, I. (2015). 'Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)', Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, accessed 30 September 2023, https://urn.nsk.hr/urn:nbn:hr:181:824788

Vancouver

Mihić I. Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae) [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Department of biology; 2015 [cited 2023 September 30] Available at: https://urn.nsk.hr/urn:nbn:hr:181:824788

IEEE

I. Mihić, "Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)", Master's thesis, Josip Juraj Strossmayer University of Osijek, Department of biology, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:181:824788

Cite this item: https://urn.nsk.hr/urn:nbn:hr:181:824788

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Metadata

Title	Određivanje spola pomoću CHD gena kod porodice čaplji (Ardeidae)
Title (english)	Sex determination in herons (Ardeidae) using CHD gene
Author	Ivan Mihić
Mentor	Alma Mikuška (mentor)
Committee member	Ljiljana Krstin (predsjednik povjerenstva)
Committee member	Ivna Štolfa Čamagajevac (član povjerenstva)
Committee member	Alma Mikuška (član povjerenstva)
Granter	Josip Juraj Strossmayer University of Osijek Department of biology Osijek
Defense date and country	2015-09-28, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Spolni dimorfizam kod mladih jedinki ĉaplji nije izražen, te je jedna od pouzdanih metoda utvrđivanja spola mladih ptica putem molekularnih biljega. Cilj diplomskog rada je usporedba metoda za izolaciju DNA iz pera i ljuski jaja ĉaplji, te utvrđivanje spola prstenovanih jedinki ĉaplji. Rezultati pokazuju da je najveći prinos DNK dobiven iz pera koja su u razvoju (prokrvljena pera) i ljuski jaja. Prinos DNA je bio manji kad se koristio komercijalni kit za izolaciju u odnosu na izolaciju standardnim protokolom. Određen je spol deset prstenovanih ĉaplji na ribnjacima Jasinje. U dva gnijezda ĉaplje dangube (Ardea purpurea) utvrđeno je ukupno šest mužjaka(tri po gnijezdu), dok su u dva gnijezda velike bijele ĉaplje (Ardea alba) utvrđene ĉetiri ženke, tri iz jednog i jedna iz drugog gnijezda. Ovo je prvo utvrđivanje spola ĉaplji putem CHD gena u Hrvatskoj. Opsežnijim višegodišnjim istraživanjima mogao bi se utvrditi odnos spolova u populaciji, stabilnost populacije, te poduzeti potrebne mjere zaštite gnijezdeće populacije ĉaplji u Hrvatskoj.
Abstract (english)	In sexually monomorphic species like herons one of the most reliable method of sex determination in young birds are molecular markers. The aim this work is a comparison of methods for the isolation of DNA from feathers and eggshells in herons, and sex determination of ringed young birds. Results showed that the highest yield of DNA was obtained from the young chick feathers, which are in development, and egg shells. The yield of DNA was lower when a commercial kit was used for isolation compared to the isolation with the standard protocol. Sex-typing was performed in ten ringed herons in the fishponds Jasinje. In two nests of purple heron(Ardea purpurea) a total of six males (three per nest) were found while in two nests of the great white egret(Ardea alba) we identified four females, three in one and one in another nest. This is the first time in Croatia that molecular approach was used for sex determination in herons by using CHD gene. More extensive research trough years would determine sex ratio in the population, population stability, and to lead to the necessary measures in order to protect nesting population of herons in Croatia.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:181:824788
Study programme	Title: Master of Science Graduate University Study Programme in Biology; specializations in: Master of Biology Course: Master of Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Department of biology, Josip Juraj Strossmayer University of Osijek
Created on	2017-01-24 09:00:10