Objectives: To determine the presence of the burnout syndrome among the nursing staff at Koprivnica General Hospital and to determine to what extent it correlates with formal education levels, age, gender, length of service and place of residence. Study design: Cross-sectional survey. Participants and methods: 146 nurses employed at Koprivnica General Hospital took part in the survey. Research instruments were general sociodemographic data and a questionnaire on professional burnout. The survey was carried out during the months of August and September 2017. Results: According to the values of emotional exhaustion, 16 (11%) participants show a low level of emotional exhaustion, 58 (39.7%) show moderate levels and 72 (49.3%) participants show a high level of emotional exhaustion. There is no significant difference in the level of emotional exhaustion related to gender, age, education level and length of service. Participants who live in town, show a significantly higher level of emotional exhaustion compared to those living in the country (P = 0.03). A low level of depersonalisation was shown by 28 (19.2%) participants, while 91 (62.3%) of them show a moderate level. Depersonalisation was significantly more present among male participants (P = 0.005) and nurses who finished four or five years of secondary education (P = 0.03), whereas there are no significant differences regarding other parameters. Significant differences in personal accomplishment related to general data about the participants have not been registered. Conclusion: The burnout syndrome is present among the nursing staff at Koprivnica General Hospital. There is a correlation between the burnout syndrome and the level of formal education, gender and place of residence. Statistically, age and length of service do not significantly affect the occurrence of the burnout syndrome.