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undergraduate thesis
Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi
Split: University of Split, University Department of Health Studies, 2019. urn:nbn:hr:176:333405
Urlić, Marta
University of Split
University Department of Health Studies

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Urlić, M. (2019). Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi (Undergraduate thesis). Split: University of Split, University Department of Health Studies. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić, Marta. "Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi." Undergraduate thesis, University of Split, University Department of Health Studies, 2019. https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić, Marta. "Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi." Undergraduate thesis, University of Split, University Department of Health Studies, 2019. https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić, M. (2019). 'Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi', Undergraduate thesis, University of Split, University Department of Health Studies, accessed 29 May 2023, https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić M. Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi [Undergraduate thesis]. Split: University of Split, University Department of Health Studies; 2019 [cited 2023 May 29] Available at: https://urn.nsk.hr/urn:nbn:hr:176:333405

M. Urlić, "Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi", Undergraduate thesis, University of Split, University Department of Health Studies, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:176:333405

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Metadata
TitleKomercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi
Title (english)COMMERCIAL STR KITS: VALUE AND CONTRIBUTION IN A FORENSIC DNA ANALYSIS
AuthorMarta Urlić
MentorDavorka Sutlović (mentor)
Committee memberDavorka Sutlović (predsjednik povjerenstva)
Committee memberIrena Drmić Hofman (član povjerenstva)
Committee memberDaniela Šupe-Domić (član povjerenstva)
GranterUniversity of Split
University Department of Health Studies
Split
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Ciljevi rada: Prikazati identifikacijske STR setove te usporediti njihovu vrijednost i značajnost u komercijalne svrhe. Metode: Opisano je pet različitih STR sustava koji se koriste u forenzičnoj DNA analizi u različite svrhe. Sadržaj svakog seta omogućuje izvođenje PCR metode pri čemu se umnaža DNA iz raznih bioloških uzoraka te se amplificirani produkti razdvajaju pa detektiraju i naposlijetku analiziraju. Rezultati: MiniFiler set reagensa najbolje se koristi u analizi malih količina DNA, asadrži 8 različitih lokusa za umnažanje DNA. SGM Plus identifikacijski kit sadrži 2 lokusa više, što doprinosi većoj osjetljivosti analize. Identifiler Plus i Power Plex koriste čak 15 STR lokusa te jedan spolno determinirajući marker – Amelogenin, što dodatno doprinosi njihovoj iznimnoj snazi diskriminacije. Zaključci: Otkriće komercijalnih STR-sustava označilo je prekretnicu u forenzičnoj DNA analizi, a uvođenje sve više lokusa u pojedine setove rezultiralo je povećanjem njihove snage diskriminacije i na taj način svelo pojavnost pogrešaka na minimum.
Abstract (english)
Aim: To present STR kits for forensic identification and compareits value and relevance for commercial purposes. Methods: Five different STR kits in use for forensic DNA analyis have been described. The content of the each kit enables the performance of PCR amplification of a DNA from different sources. The amplified products were separated and set up for detection and interpretation. Results: The MiniFiler kit was the best used, especially in analysis of the small amonuts of degraded DNA, and amplified 8 different loci. Two more loci are included in the SGM Plus identification kit which results in a much higher sensitivity rate. The Identifiler Plus and the PowerPlex 16 amplification kits amplify 15 different STR loci and one gender-determining marker – Amelogenin, which strongly contributes to a greater power of discrimination. Conclusions: Discovery of the commercial STR-kits marked a turning point in the forensic DNA analysis, and by including more and more loci in each set has enhanced the power of discrimination, which led to a error rate reduction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:333405
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of the University Department for Health Studies, University of Split
Created on2020-06-23 09:03:29

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