Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva

Hasanović, Andrea

prikaz prve stranice dokumenta Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva

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master's thesis

Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:547044

Hasanović, Andrea

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

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APA 6th Edition

Hasanović, A. (2011). Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:547044

MLA 8th Edition

Hasanović, Andrea. "Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:547044

Chicago 17th Edition

Harvard

Hasanović, A. (2011). 'Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:547044

Vancouver

Hasanović A. Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2025 January 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:547044

IEEE

A. Hasanović, "Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:547044

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Title	Karakterizacija autohtonih Enterococcus vrsta izoliranih iz tradicionalno proizvedenih sireva
Title (english)	Characterization of autochthonous Enterococcus species isolated from traditionally produced cheeses
Author	Andrea Hasanović
Mentor	Jagoda Šušković (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology) Zagreb
Defense date and country	2011-12, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	U ovom radu provedena je izolacija autohtonih bakterija mliječne kiseline (BMK) iz tradicionalno proizvedenih sireva s ciljem odabira predstavnika Enterococcus vrsta kao potencijalnih probiotičkih starter kultura. Pomoću klasičnih mikrobioloških metoda odabrani autohtoni izolati su okarakterizirani kao gram-pozitivni, katalaza-negativni, nesporogeni, kuglasti, koji uspješno spuštaju pH vrijednost hranjive podloge, te sintetiziraju visoke koncentracije mliječne kiseline. Provedena je genotipska identifikacija sekvencioniranjem 16S rRNA gena prema kojoj je među odabranim autohtonim BMK bilo prisutno 7 različitih bakterijskih sojeva iz roda Enterococcus. Autohtoni sojevi BMK BGRE 2-24, BGRE 2-43 i BGBU 1-42 najbolje preživljavaju simulirane uvjete gastrointestinalnog trakta. Ispitivanjem osjetljivosti Enterococcus vrsta na 12 različitih antibiotika, utvrđeno je da Enterococcus faecalis / durans / faecium BGBU 1-41 nije osjetljiv na neomicin i streptomicin, kao i sojevi Enterococcus faecalis BGRE 2-24 i Enterococcus faecium/ durans BGRE 2-57, dok Enterococcus faecium / durans BGBU 1-42 i Enterococcus hirae / faecium BGRE 2-43 nisu osjetljivi na streptomicin, ali jesu na neomicin koji također pripada skupini aminoglikozidnih antibiotika.
Abstract (english)	In this paper isolation of the autochthonous isolates of lactic acid bacteria (LAB) from traditionally produced cheeses was performed aimed to select representatives of Enterococcus species with a potential as probiotic starter cultures. Selected autochthonous isolates were characterized as gram-positive, catalase-negative sphere-shaped bacteria by conventional microbiological methods, which were successfull in lowering pH value of the medium and synthesized high concentrations of lactic acid. Identification performed by 16S rRNA gene sequencing revealed that between selected autochthonous LAB 7 different strains were identified as representatives of Enterococcus. Autochthonous LAB BGRE 2-24, BGRE 2-43 and BGBU 1-42 showed high survival rates in simulated gastrointestinal tract conditions. Investigation of the susceptibility to different antibiotics revealed that Enterococcus faecalis / durans / faecium BGBU 1-41, Enterococcus faecalis BGRE 2-24 and Enterococcus faecium / durans BGRE 2-57 were not sensitive to neomycin and streptomycin while strain Enterococcus faecium / durans BGBU 1-42 and Enterococcus hirae / faecium BGRE 2-43 were resistant to neomycin but not to streptomycin which are all aminoglycoside antibiotics.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:547044
Study programme	Title: Biotechnology; specializations in: Biochemical Engineering Course: Biochemical Engineering Study programme type: university Study level: graduate Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
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Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2017-06-01 09:20:29