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master's thesis
Stabilnost genoma bakterije Escherichia coli nakon gama zračenja
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:628110
Ćosić, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Cite this document

Ćosić, I. (2010). Stabilnost genoma bakterije Escherichia coli nakon gama zračenja (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:628110

Ćosić, Iva. "Stabilnost genoma bakterije Escherichia coli nakon gama zračenja." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:628110

Ćosić, Iva. "Stabilnost genoma bakterije Escherichia coli nakon gama zračenja." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:628110

Ćosić, I. (2010). 'Stabilnost genoma bakterije Escherichia coli nakon gama zračenja', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:628110

Ćosić I. Stabilnost genoma bakterije Escherichia coli nakon gama zračenja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:628110

I. Ćosić, "Stabilnost genoma bakterije Escherichia coli nakon gama zračenja", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:628110

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Metadata
TitleStabilnost genoma bakterije Escherichia coli nakon gama zračenja
Title (english)Genome stability in Escherichia coli bacterium after gamma radiation
AuthorIva Ćosić
MentorDavor Zahradka (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2010-02-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Protein RecA je esencijalni protein homologne rekombinacije bakterije E. coli. Stanice mutirane u genu recA imaju smanjenu vijabilnost. U ovom radu je proučena stabilnost genoma nakon γ-zračenja u stanicama divljeg tipa, te u mutantima bakterije E. coli. Istraživanja su pokazala da mutanti (recA ΔrecBC i recAΔ recBCΔ sbcB) preživljavaju manje doze zračenja od divljeg tipa bakterije te da dolazi do rearanžmana samo u stanicama divljeg tipa. Za analizu genoma koristila se gel elektroforeza u promjenjivom električnom polju (metoda PFGE).
Abstract (english)
RecA protein is an essential protein of the homologous recombination of the E. coli bacterium. Cells mutated in the recA gene have a lowered viability. In this work, the stability of the genome after γ- radiation in wild type cells was studied as well as mutants of the bacterium E. coli. Studies have shown that mutants (recA ΔrecBC i recAΔ recBCΔ sbcB) survive smaller doses of radiation than the wild type bacteria and genome rearrangements have been detected only in the wild type cells. Pulsed-field gel electrophoresis (PFGE) was used for genome analysis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:628110
Study programmeTitle: Biotechnology; specializations in: Biochemical Engineering
Course: Biochemical Engineering
Study programme type: university
Study level: graduate
Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2017-08-28 11:11:41

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