Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i                                                                                  testa lomljivosti kromatida

Lončar, Jovica

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master's thesis

Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2005. urn:nbn:hr:159:444060

Lončar, Jovica

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

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APA 6th Edition

Lončar, J. (2005). Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:444060

MLA 8th Edition

Lončar, Jovica. "Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2005. https://urn.nsk.hr/urn:nbn:hr:159:444060

Chicago 17th Edition

Harvard

Lončar, J. (2005). 'Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:444060

Vancouver

Lončar J. Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2005 [cited 2025 January 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:444060

IEEE

J. Lončar, "Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2005. Available at: https://urn.nsk.hr/urn:nbn:hr:159:444060

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:444060

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Title	Procjena učinka pušenja na genom ljudskih limfocita izloženih γ-zračenju primjenom testa mikronukleusa i testa lomljivosti kromatida
Title (english)	Estimation of smoking effect on genome of human lymphocytes exposed to γ-radiation using micronucleus assay and chromatid breakage assay
Author	Jovica Lončar
Mentor	Verica Garaj-Vrhovac (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Biology and Microbial Genetics) Zagreb
Defense date and country	2005-05-06, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Primjenom testa mikronukleusa i testa lomljivosti kromatida istraženi su genotoksički učinci pušenja na genom ljudskih limfocita u spontanim in vivo uvjetima i γ-zračenjem induciranim in vitro uvjetima. Nadalje je učinjena procjena utjecaja spola na oštećenost genoma te procjena razlika u radioosjetljivosti. Testom mikronukleusa utvrđen je statistički značajno veći stupanj oštećenja genoma pušača od stupnja oštećenja genoma nepušača. Utjecaj spola nije pronađen. Testom lomljivosti kromatida utvrđeno je da se broj lomova po stanici, kao i broj stanica s lomom, između pušača i nepušača statistički značajno ne razlikuju. Velike individualne razlike u radioosjetljivosti dobivene testom mikronukleusa nisu potvrđene testom lomljivosti kromatida. Stoga se ne može izvesti jednoznačan zaključak.
Abstract (english)	Using micronucleus assay and chromatid breakage assay we investigated genotoxicity of smoking on genome of human lymphocytes in spontaneous in vitro conditions and γ-radiation induced in vivo conditions. Furthermore we estimated the sex effect in damaging the genome and estimated the differences in radiosensitivity. Micronucleus assay established that the degree of damage of genome in smokers’ lymphocytes was higher compared to nonsmokers and the difference reached statistical significance. Sex effect was not found. Chromatid breakage assay didn’t find statistically significant difference in breaks per cell, as well as in number of cells with break, between smokers and nonsmokers. Big interindividual differences in radiosensitivity established with micronucleus assay were not confirmed by chromatid breakage assay. Therefore we can not make univocal conclusion.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:444060
Study programme	Title: Biotechnology; specializations in: Biochemical-Microbiology Course: Biochemical-Microbiology Study programme type: university Study level: graduate Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki (diplomirani inženjer biotehnologije; smjer biokemijsko-mikrobiološki)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
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Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2017-09-12 07:44:01