Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae

Debanić, Fran

prikaz prve stranice dokumenta Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae

undergraduate thesis

Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:028171

Debanić, Fran

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Cite this document

APA 6th Edition

Debanić, F. (2018). Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:028171

MLA 8th Edition

Debanić, Fran. "Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:028171

Chicago 17th Edition

Harvard

Debanić, F. (2018). 'Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:028171

Vancouver

Debanić F. Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2025 February 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:028171

IEEE

F. Debanić, "Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:028171

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:028171

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Metadata

Title	Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae
Title (english)	Heterologous expression of the atrazine degradation pathway enzymes on the surface of yeast Saccharomyces cerevisiae cell
Author	Fran Debanić
Mentor	Renata Teparić (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Chemistry and Biochemistry) (Laboratory for Biochemistry) Zagreb
Defense date and country	2018-09-19, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Kvasac Saccharomyces cerevisiae jedan je od najčešće korištenih mikroorganizama u biotehnologiji, biomedicini i raznim znanstvenim istraživanjima. U ovom radu kvasac se koristio za ekspresiju enzima za razgradnju atrazina koji potječu iz Pseudomonas sp. ADP. Atrazin je herbicid koji je zabranjen od strane EU jer zbog svog kumulativnog efekta izaziva onečišćenje okoliša. Metabolički put razgradnje atrazina se sastoji od šest enzimskih reakcija od kojih je svaka katalizirana s po jednim enzimom. Cilj rada je bio eksprimirati i imobilizirati na površini stanične stijenke kvasca S. cerevisiae prva četiri enzima metaboličkog puta razgradnje atrazina (AtzA, AtzB, AtzC i AtzD), a u tu svrhu korišteni su proteini stanične stijenke kvasca Pir4 i Ccw12 s kojima su ti enzimi bili genetički fuzionirani. Uspješnost ekspresije i imobilizacije rekombinantih proteina provjeravala se Western blot analizom proteina ekstrahiranih iz izoliranih staničnih stijenki kvasca. Osim toga ispitivala se i aktivnost eksprimiranih rekombinantih enzima u tekućem mediju i na krutoj podlozi u Petrijevim zdjelicama.
Abstract (english)	Yeast Saccharomyces cerevisiae is one of the most commonly used microorganisms in biotechnology, biomedicine and various scientific research. In this thesis yeast was used to express the atrazine degradation enzymes originated from Pseudomonas sp. ADP. Atrazine is a herbicide that is banned from the EU because of its cumulative effect on environmental pollution. The metabolism of atrazine degradation consists of six enzymatic reactions each of which is catalysed with one enzyme. The aim of this study was to express and immobilize the first four enzymes of the atrazine degradation pathway (AtzA, AtzB, AtzC and AtzD) on the surface of the S. cerevisiae cell. For this purpose, Pir4 and Ccw12 yeast cell wall proteins were genetically fused with these enzymes. Success of expression and immobilization of the recombinant proteins was verified by Western blot analysis of proteins extracted from isolated yeast cell walls. In addition, the activity of expressed recombinant enzymes in the liquid medium and on the solid substrate in Petri dishes was also investigated.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:028171
Study programme	Title: Biotechnology Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2018-09-24 07:56:17