In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša

Moslavac, Leonina

prikaz prve stranice dokumenta In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša

master's thesis

In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:032637

Moslavac, Leonina

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Cite this document

APA 6th Edition

Moslavac, L. (2019). In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:032637

MLA 8th Edition

Moslavac, Leonina. "In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:032637

Chicago 17th Edition

Harvard

Moslavac, L. (2019). 'In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 08 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:032637

Vancouver

Moslavac L. In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2025 February 08] Available at: https://urn.nsk.hr/urn:nbn:hr:159:032637

IEEE

L. Moslavac, "In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:032637

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:032637

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Title	In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost 5-fluorouracila na tumorske stanice mliječne žlijezde miša
Title (english)	In vitro study of the adjuvant effect of quisinostat on the cytotoxicity of 5-fluorouracile on mouse mammary gland tumor cells
Author	Leonina Moslavac
Mentor	Lidija Šver (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Biology and Microbial Genetics) Zagreb
Defense date and country	2019-09-25, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Tumori su osnovni predmet istraživanja velike većine eksperimentalnih radova zbog pojave rastuće smrtnosti, neovisno o dobi i spolu. Tumor dojke najzastupljeniji je oblik kod ženske populacije te je kao takav predmet istraživanja ovog rada, s ciljem ispitivanja osjetljivosti stanica tumora mliječne žlijezde miša na primijenjeni lijek 5-fluorouracil (5-FU) te pomoćno sredstvo quisinostat (inhibitor histon deacetilaza), u obliku 2D i 3D staničnih kultura. Pritom, odreĎena je koncentracija lijeka koja daje približno 50% preživljenja stanica te efikasnost istovremenog tretmana s 5-FU i qusinostatom te predtretmana qusinostatom uz dodatak lijeka. Ispitan je utjecaj različitih koncentracija lijeka 5-FU te koncentracije qusinostata. Za odreĎivanje 2D staničnih kultura korišten je resazurin test, dok su za 3D stanične kulture korišteni CellTox™ Green Cytotoxicity, RealTime-Glo MT Cell Viability i CellTiter-Glo 3D Cell Viability test. Zaključno, 3D stanična kultura pokazala je veću osjetljivost na istovremeni tretman u odnosu na 2D staničnu kulturu, koji se pokazao kao najbolja opcija primjene u tretiranju stanica tumora mliječne žlijezde miša.
Abstract (english)	Tumors are the main subject of research in the vast majority of experimental studies due to the onset of increasing mortality, regardless of age and gender. Breast cancer is the most prevalent form in the female population, and as such is the subject of research in this study, with the aim of examining the sensitivity of mouse mammary gland tumor cells to the drug 5-fluorouracile (5-FU) and the adjuvant quisinostat (histone deacetylase inhibitor), in the form of 2D and 3D cell cultures. The concentration of the drug giving approximately 50% cell survival and the efficacy of combination treatment with 5-FU and quisinostat and pretreatment with qusinostat with drug addition were determined. The effect of different concentrations of 5-FU and the concentrations of qusinostat were examined. Resazurin assay was used to determine viability in 2D cell cultures, whereas CellTox ™ Green Cytotoxicity, RealTime-Glo MT Cell Viability and CellTiter-Glo 3D Cell Viability Assay were used for 3D cell cultures. To conclude, 3D cell culture showed greater sensitivity to combination treatment than 2D cell culture, which has proven to be the best application option in the treatment of mouse mammary gland tumor cells.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:032637
Study programme	Title: Molecular Biotechnology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2020-02-13 11:59:07