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master's thesis
Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:389678
Dobrić, Nina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for General Microbiology and Food Microbiology

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Dobrić, N. (2019). Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:389678

Dobrić, Nina. "Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:389678

Dobrić, Nina. "Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:389678

Dobrić, N. (2019). 'Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:389678

Dobrić N. Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2025 February 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:389678

N. Dobrić, "Antibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:389678

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Metadata
TitleAntibakterijsko djelovanje citrinina na odabrane patogene bakterije i Gluconobacter oxydans
Title (english)Antibacterial activity of citrinin against selected pathogenic bacteria and Gluconobacter oxydans
AuthorNina Dobrić
MentorKsenija Markov (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for General Microbiology and Food Microbiology)
Zagreb
Defense date and country2019-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Citrinin je mikotoksin kojeg proizvode neke vrste plijesni iz roda Penicillium, ali i plijesni iz rodova Aspergillus i Monascus. Zbog svoje kemijske strukture citrinin posjeduje širok raspon toksičnih učinaka i njegova prisutnost predstavlja opasnost po zdravlje ljudi i životinja. Cilj ovog rada bio je ispitati antibakterijska svojstva citrinina na najčešće patogene mikroorganizme, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus te nepatogenu bakteriju Gluconobacter oxydans. Antibakterijsko djelovanje citrinina određeno je disk difuzijskom metodom, mjerenjem promjera zona inhibicije rasta na čvrstim hranjivim podlogama u Petrijevim zdjelicama te turbidimetrijskom metodom u mikrotitarskim pločicama. Također, praćen je broj živih bakterija tijekom izloženosti različitim koncentracijama citrinina. Rezultati dobiveni disk difuzijskom metodom pokazali su inhibicijsko djelovanje koncentracije citrinina od 200 mg mL-1 na sve ispitivane mikroorganizme, a niža koncentracija (100 mg mL-1) nije spriječila rast E. coli i S. typhimurium. Turbidimetrijskom metodom, kao i praćenjem broja živih bakterija, dokazana je antibakterijska aktivnost dviju koncentracija citrinina.
Abstract (english)
Citrinin is a mycotoxin produced by several species of molds of the Penicillium genera, but also Aspergillus and Monascus genera. Due to its chemical structure, citrinin has a wide toxic effect and its presence poses a risk to human and animal health. The aim of this study was to determine antibacterial properties of citrinin against selected pathogenic microorganisms, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Listeria monocytogenes, Staphylococcus aureus and the non-pathogenic Gluconobacter oxydans. Antibacterial activities of citrinin was determined by disk diffusion method, measuring diametar of inhibition zone on solid media in Petri dishes and turbidimetric method in microtitre plates. Also, number of living bacteria was monitored during the exposure of different concentrations of citrinin. The results of the disk diffusion method showed an inhibitory effect of citrinin in concentration of 200 μg mL-1 on all tested microorganisms, while a lower concentration (100 μg mL-1) did not prevent the growth of E. coli and S. typhimurium. Turbidimetric method, as well as monitoring number of living bacteria, proved antibacterial activities of citrinin in both concentrations.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:389678
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
RepositoryRepository of the Faculty of Food Technology and Biotechnology
Created on2020-02-13 14:39:18

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