Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN

Slišković, Ana

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undergraduate thesis

Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. urn:nbn:hr:159:057005

Slišković, Ana

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

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APA 6th Edition

Slišković, A. (2020). Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:057005

MLA 8th Edition

Slišković, Ana. "Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2020. https://urn.nsk.hr/urn:nbn:hr:159:057005

Chicago 17th Edition

Harvard

Slišković, A. (2020). 'Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:057005

Vancouver

Slišković A. Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2020 [cited 2025 February 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:057005

IEEE

A. Slišković, "Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:159:057005

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:057005

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Title	Restrikcijska analiza plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN
Title (english)	Restriction analysis of plasmids pRS40cN, pRS52cN, pRS53cN and pRS55cN
Author	Ana Slišković
Mentor	Ivan-Krešimir Svetec (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Biology and Microbial Genetics) Zagreb
Defense date and country	2020-09-01, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Pod pojmom restrikcijska analiza, u molekularnoj genetici i genetičkom inženjerstvu, podrazumijeva se analiza DNA korištenjem restrikcijskih enzima, odnosno endonukleaza koje cijepaju DNA u točno određenim sekvencijama. Stoga su ovi enzimi nezamjenjivi u genetičkom inženjerstvu i mnogim molekularno-genetičkim metodama koje imaju primjenu u dijagnostici, filogeniji i identifikaciji organizama. U ovom radu je restrikcijskom analizom potvrđena struktura prethodno konstruiranih plazmida pRS40cN, pRS52cN, pRS53cN i pRS55cN. U tu su svrhu plazmidi najprije izolirani i pročišćeni iz velikih volumena kultura bakterije Eschericha coli, a zatim pocijepani odgovarajućim restrikcijskim enzimima te podvrgnuti elektroforezi u agaroznom gelu. Na temelju rezultata analize potvrđeno je da restrikcijske mape svih plazmida odgovaraju njihovoj stvarnoj strukturi.
Abstract (english)	In molecular genetics and genetic engineering, the term restriction analysis refers to the analysis of DNA molecules using restriction enzymes, endonucleases that cleave DNA in specific nucleotide sequences. Therefore, these enzymes are indispensable in genetic engineering, as well as in many molecular genetic methods that are used in diagnostics, phylogeny and identification of organisms. In this paper, restriction analysis was used to confirm the structure of previously constructed plasmids pRS40cN, pRS52cN, pRS53cN and pRS55cN. For this purpose, the plasmids were first isolated and purified from large volumes of Escherichia coli cultures, then digested with appropriate restriction enzymes and subjected to agarose gel electrophoresis. Based on the results of the analysis, it was confirmed that the restriction maps of all plasmids correspond to their actual structure.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:057005
Study programme	Title: Biotechnology Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2020-10-16 11:22:39