Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata

Štanfel, Lana

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master's thesis

Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. urn:nbn:hr:159:332334

Štanfel, Lana

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

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APA 6th Edition

Štanfel, L. (2024). Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:332334

MLA 8th Edition

Štanfel, Lana. "Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2024. https://urn.nsk.hr/urn:nbn:hr:159:332334

Chicago 17th Edition

Harvard

Štanfel, L. (2024). 'Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 08 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:332334

Vancouver

Štanfel L. Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2024 [cited 2025 February 08] Available at: https://urn.nsk.hr/urn:nbn:hr:159:332334

IEEE

L. Štanfel, "Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:159:332334

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:332334

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Metadata

Title	Razvoj HPLC metode za analizu antibiotika doksiciklin hiklata i razgradnih produkata
Title (english)	Development of HPLC method for analysis of antibiotic doxycycline hyclate and its degradation products
Author	Lana Štanfel
Mentor	Antonija Trontel (mentor)
Mentor	Anita Šporec (pomoć pri izradi, neposredni voditelj)
Committee member	Mario Novak (predsjednik povjerenstva)
Committee member	Andreja Leboš-Pavunc (član povjerenstva)
Committee member	Blaženka Kos (član povjerenstva)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology) Zagreb
Defense date and country	2024-09-27, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Doksiciklin hiklat je jedan od često upotrebljavanih antibiotika za liječenje različitih bolesti kod ljudi i životinja. Stoga je neophodno ispuniti zahtjeve o kvaliteti lijeka kao što je mikrobiološka čistoća u cilju postizanja sigurnosti primjene lijeka. Uobičajeni pristupi inaktivacije antibiotika uključuju razrjeđivanje do niskih koncentracija ili kemijsku razgradnju, dok se u ovom radu istražuje enzimska razgradnja kao ekološki prihvatljivija alternativa. Odabran je enzim lakaza koji oksidacijom fenolnih skupina razgrađuje doksiciklin hiklat prisutan u farmaceutskom proizvodu odnosno tableti. Razvijena je gradijentna HPLC metoda za praćenje promjene koncentracije doksiciklin hiklata i srodnih spojeva. Primjenom Luna Omega Polar C18 kolone (150 x 3 mm, 3 µm) s mobilnom fazom koja se sastoji od acetatnog pufera pH=8,8 i acetonitrila, uz protok 0,8 mL/min i pri 274 nm, eluira se doksiciklin hiklat i njegovi razgradni spojevi unutar 25 minuta. Metodom se može učinkovito pratiti enzimska razgradnja, što je ključno za procjenu djelotvornosti lakaze u razgradnji antibiotika.
Abstract (english)	Doxycycline hyclate is one of the most commonly used antibiotics for treating various diseases in humans and animals. Therefore, it is essential to meet the quality requirements of the drug, such as microbiological purity, to ensure the safety of its application. Traditional approaches to antibiotic inactivation include dilution to low concentrations or chemical degradation, while this study investigates enzymatic degradation as a more environmentally friendly alternative. The enzyme laccase, which oxidizes phenolic groups, was selected to degrade doxycycline hyclate present in a pharmaceutical product (tablet). A gradient HPLC method was developed to monitor changes in the concentration of doxycycline hyclate and related compounds. Using a Luna Omega Polar C18 column (150 x 3 mm, 3 µm) with a mobile phase consisting of acetate buffer pH=8.8 and acetonitrile, at a flow rate of 0.8 mL/min and detection at 274 nm, doxycycline hyclate and its degradation products elute within 25 minutes. This method can effectively monitor the enzymatic degradation, which is crucial for assessing the effectiveness of laccase in the degradation of the antibiotic.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:332334
Study programme	Title: Bioprocess Engineering Study programme type: university Study level: graduate Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2024-10-17 12:13:46