Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica

Stjepanović, Josipa

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master's thesis

Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. urn:nbn:hr:163:824421

Stjepanović, Josipa

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of pharmaceutical technology

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APA 6th Edition

Stjepanović, J. (2018). Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:824421

MLA 8th Edition

Stjepanović, Josipa. "Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2018. https://urn.nsk.hr/urn:nbn:hr:163:824421

Chicago 17th Edition

Harvard

Stjepanović, J. (2018). 'Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 23 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:824421

Vancouver

Stjepanović J. Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2018 [cited 2025 January 23] Available at: https://urn.nsk.hr/urn:nbn:hr:163:824421

IEEE

J. Stjepanović, "Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:163:824421

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:824421

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Metadata

Title	Ispitivanje kriozaštitnog učinka komercijalno dostupnih medija za pohranu rožnica
Title (english)	An examination of the cryoprotective effect of commercially available preservation media
Author	Josipa Stjepanović
Mentor	Ivan Pepić (mentor)
Committee member	Ivan Pepić (predsjednik povjerenstva)
Committee member	Dubravka Vitali Čepo (član povjerenstva)
Committee member	Višnja Drinovac (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of pharmaceutical technology) Zagreb
Defense date and country	2018-09-21, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Sve raširenijom potrošnjom oftalmičkih lijekova dolazi do povećane potrebe za pretkliničkim istraživanjima lijekova. Nedostaci ex vivo modela rožnice u svrhu ispitivanja permeabilnosti djelatne tvari preko barijere rožnice su osiguravanje primjerenog broja animalnih rožnica te brzo provođenje pokusa. Budući da je epitel glavna barijera prijenosu djelatnih tvari, a standardnom pohranom humanih rožnica stvaraju se velika epitelna oštećenja, takva pohrana nije primjenjiva za ispitivanja permeabilnosti na animalnim rožnicama. Cilj ovog diplomskog rada je ispitati primarnu i sekundarnu zaštitu rožnica kriopohranjenih u složenim medijima kao kriozaštitnim sredstvima korištenjem različitih protokola zamrzavanja i odmrzavanja. Primjereno kriozaštićene rožnice mogu poslužiti kao vrijedan alat za pretklinička istraživanja. Očuvanost i integritet epitelne barijere rožnice procijenjeni su pomoću vrijednosti izmjerenog transepitelnog električnog otpora. Rezultati ukazuju na oštećenje i narušavanje epitelne barijere tijekom 48 sati kriopohrane u komercijalno dostupnim medijima za pohranu rožnice, CorneaMax® i CorneaJet®. Najslabija primarna zaštita opažena je provođenjem protokola zamrzavanja u tekućem dušiku pri -196°C, dok su više vrijednosti opažene postupnim zamrzavanjem rožnica. Medij CorneaJet® kao kriozaštitno sredstvo pokazao je bolju ukupnu sekundarnu zaštitu rožnica tijekom kriopohrane u odnosu na medij CorneaMax®.
Abstract (english)	The increased use of ophthalmic medications leads to an increased demand for preclinical drug research. The disadvantages of the use of ex vivo corneal model for examining the permeability of active substance through corneal barrier are ensuring an adequate number of animal corneas and the immediate conduct of the experiment. Since epithelium is the main barrier to the transfer of active substances, and the standard storage of human corneas causes serious epithelial damage, that type of storage is not suitable for permeability tests on animal corneas. The aim of this thesis is to examine the primary and secondary protection of cornea cryopreserved in complex media as cryoprotective agents, by using different freezing and thawing protocols. Properly cryoprotected corneas can be used as a valuable tool for preclinical research in ophthalmology. The preservation and integrity of epithelial barrier were estimated by measuring the transepithelial electrical resistance. The results indicate damage and deterioration of the epithelial barrier during 48 hours of cryopreservation in two commercially available corneal storage media, CorneaMax® i CorneaJet®. The lowest primary protection has been proved by the application of the liquid nitrogen freezing protocol at -196°C, while higher values have been obtained by gradual freezing. CorneaJet® medium has shown better overall secondary protection as a cryoprotective agent compared to CorneaMax® medium.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:824421
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2019-09-06 15:03:32