Tumor cells are characterized by the imbalance of basic cellular regulation mechanisms, and the result is abnormal proliferation, uncontrolled growth, and division. The disbalance between oxidative and antioxidative mechanisms in favor of oxidation is called oxidation stress. This process creates free radicals. The presence of free radicals can cause lipid peroxidation, damage to DNA and proteins, stimulation of cytotoxic actions, induction of mutations, chromosomal aberrations, which will eventually lead to tumor development. In this paper, the antioxidant activity of 10 newly synthetic compounds were examined. Synthesized compounds are derivatives of itaconic acid and anti-malarial drugs: primaquine, chloroquine and mefloquine, as well as conjugates of itaconic acid and fluoroaniline, pyridine and indole. They are synthesized as potential anticancer and antiviral agents. A DPPH method was chosen to assess antioxidant activity quickly and easily in more complex biological samples. The DPPH method was established using two known compounds with antioxidant activity, vitamin C and Trolox. The standards and test compounds were dissolved in the DMSO solvent to a concentration of 50 mM. The stock solutions were then prepared in ethanol in a dilute series (1 mM - 0.5 nM). They were measured for absorbance and the value of antioxidant activity was calculated in relation to positive control. The examined itaconic acid conjugates in the DPPH test showed no antioxidant activity. Their IC50 values were greater than 1000 μM.