Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji

Šalov, Mirela

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master's thesis

Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. urn:nbn:hr:163:412908

Šalov, Mirela

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of medical biochemistry and haematology

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APA 6th Edition

Šalov, M. (2021). Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:412908

MLA 8th Edition

Šalov, Mirela. "Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2021. https://urn.nsk.hr/urn:nbn:hr:163:412908

Chicago 17th Edition

Harvard

Šalov, M. (2021). 'Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 19 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:412908

Vancouver

Šalov M. Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2021 [cited 2025 January 19] Available at: https://urn.nsk.hr/urn:nbn:hr:163:412908

IEEE

M. Šalov, "Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:163:412908

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:412908

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Title	Ispitivanje citotoksičnosti derivata itakonske kiseline kao potencijalnih citostatika na THP-1 staničnoj liniji
Title (english)	Cytotoxicity testing of itaconic acid derivatives as potential cytostatics on THP-1 cell line
Author	Mirela Šalov
Mentor	Karmela Barišić (mentor)
Mentor	Mario Matijašić (mentor)
Committee member	Karmela Barišić (predsjednik povjerenstva)
Committee member	Donatella Verbanac (član povjerenstva)
Committee member	Mario Matijašić (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of medical biochemistry and haematology) Zagreb
Defense date and country	2021-09-14, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Pharmacy
Abstract	Razvoj novoga lijeka dugotrajan je i skup proces. Nakon što se izabere bolest za koju će se razvijati novi lijek i identificira meta na koju će lijek djelovati, različitim biološkim testovima ispituje se farmakološki učinak molekula. Optimiranjem strukture lijeka poboljšavaju se farmakodinamička i farmakokinetička svojstva. Ukoliko lijek prođe pretkliničku i kliničku fazu razvoja, pokreće se postupak registracije lijeka. Tijekom razvoja koriste se brojne in vitro, in vivo i in silico metode. Testovi citotoksičnosti korisni su alati u otkrivanju potencijalnih citostatika. U tu svrhu, zbog jednostavnosti korištenja i lakoće manipuliranja, često se koriste stanične linije. Uslijed velikog broja oboljelih od malignih bolesti i neadekvatne postojeće terapije, postoji stalna potreba za razvojem učinkovitijih i selektivnijih citostatika. U ovom su radu ispitani derivati itakonske kiseline kao potencijalni citostatici. Ispitivanje je provedeno na stanicama akutne monocitne leukemije THP-1. Nakon što su stanice tretirane otopinom sintetskih spojeva u različitim koncentracijama, određena je vijabilnost stanica pomoću MTS testa. Rezultati su prikazani u obliku IC50 vrijednosti. Svi ispitivani spojevi pokazuju citotoksičnost za stanice, a najučinkovitiji je spoj MI5, konjugat itakonske kiseline i klorokina.
Abstract (english)	The development of a new drug is a time consuming and expensive process. Once the disease for which the new drug will be developed is selected and the target on which the drug will act is identified, the pharmacological effect of the molecules is examined by various biological tests. Optimizing the drug structure improves the pharmacodynamic and pharmacokinetic properties. If the drug passes the preclinical and clinical phase of development, the drug registration procedure is initiated. Numerous in vitro, in vivo and in silico methods are used during development. Cytotoxicity tests are useful tools in detecting potential cytostatics. They use cell lines due to the simplicity and ease of manipulation. Considering the large number of patients with malignant diseases and inadequate existing therapy, there is a constant need to develop more effective and selective cytostatics. This thesis analyzed itaconic acid derivatives as potential cytostatics. The study was performed on acute monocyte leukemia THP-1 cells. After the cells were treated with a solution of synthetic compounds at different concentrations, cell viability was determined by using the MTS assay. The results are presented in the form of IC50 values. All test compounds were cytotoxic for cells but the most effective compound was MI5 which is a conjugate of itaconic acid and chloroquine.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:412908
Study programme	Title: Pharmacy Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2022-02-08 14:02:44