Nebulizers are drug delivery devices most commonly used in hospital setting for the treatment of a variety of pulmonary diseases. During the last few years new nebulizer models are being developed in order to expand its use toward new treatment indications and outside the hospitals. One of the most recent types of nebulizers is the pulsating nebulizer. Its unique property is that, instead of applying a constant pressure on the nebulized aerosol, it adds pulsation to the aerosol stream. The addition of pulsation to the aerosol stream results in a deeper penetration of the nasal cavity, including penetration into the hard to reach paranasal sinuses. The applicability of pulsating nebulizators in nasal administration of innovative drug delivery systems still needs to be investigated. The aim of this study was to prepare pseudoplastic in situ gelling fluticasone propionate nanosuspensions and explore the applicability of pulsating nebulizer for their nasal delivery. In this study, in situ gelling fluticasone propionate (0,06 %, w/w) nanosuspensions with appropriate particle size and polydispersity index were successfully prepared using polysorbate 80 (0,03 %, w/w) as a wetting agent, sodium hyaluronate (0,05 %, w/w) as s constitutive polymer and pectin (0,9 %, m/m) and gellan gum (0,1%, w/w) or xanthan gum (0,05 %, w/w) as in situ gelling polymer. Both in situ gelling nanosuspensions showed pseudoplastic behaviour. Pulsating nebulisation provided drug delivery of the in situ gelling fluticasone propionate nanosuspensions at a constant velocity. At the nebulizer operating time of 20, 30 or 45 seconds appropriate dosing uniformity was achieved, regardless of the preheating time of the nebulizer. Pulsating nebulisation of in situ gelling fluticasone propionate nanosuspensions provided widespread and uniform drug deposition over the whole nasal cavity model. Nebulised aerosol reached much deeper parts of the turbinate region in relation to the same nanosuspensions delivered by conventional nasal spray. No significant difference was noticed between in situ gelling fluticasone propionate nanosuspensions containing gellan gum or xanthan gum while being administrated with pulsating nebulizer and are therefore considered equally suitable for this mode of nasal delivery.