Myasthenia gravis is an autoimmune disease that most often occurs due to the appearance of autoantibodies to acetylcholine receptor (anti-AChR) and muscle-specific tyosine kinase (anti-MuSK), which causes impaired neuromuscular transmission resulting in muscle weakness and fatigue. The aim of this study was to conduct a verification procedure for two new methods before their implementation into routine work in the Department of Clinical Chemistry, Sestre milosrdnice University Hospital. Tested methods were the quantitative ELISA method for the determination of anti-AChR antibodies and the qualitative method of indirect immunofluorescence (IIF) on transfected cells for the determination of anti-MuSK antibodies. The imprecision of the ELISA method for anti-AChR was tested by measuring control samples. The imprecision did not meet the criteria declared by the manufacturer, but meets the one of clinical interpretation as well as the generally accepted criterion of imprecision of ELISA methods. The trueness of the ELISA method for anti-AChR was tested by comparison with the identical method implemented in the Department of Laboratory Diagnostics, University Hospital Centre Zagreb, based on 41 serum samples. A perfect agreement in the categorization of the results between the methods was established and regression analysis revealed that there is no statistically significant difference between the methods in the range of clinically significant results. The reproducibility of the IIF method for the detection of anti-MuSK antibodies was tested on 20 repetition results of negative and positive control samples and met the acceptance criteria according to CLSI EP12-A2. The trueness of the IIF was tested by comparison with the ELISA method implemented in the Department of Laboratory Diagnostics, University Hospital Centre Zagreb, based on 33 serum samples. A satisfactory comparability was established, but due to the small number of positive samples (low incidence of anti-MuSK antibodies), the precondition for the correct interpretation of the statistical test was not met. The results of the verification confirm that the tested methods are acceptable for implementation into routine work.