Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina

Ćurčić, Matea

prikaz prve stranice dokumenta Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina

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master's thesis

Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina

Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. urn:nbn:hr:163:882018

Ćurčić, Matea

University of Zagreb
Faculty of Pharmacy and Biochemistry
Department of biochemistry and molecular biology

Cite this document

APA 6th Edition

Ćurčić, M. (2016). Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina (Master's thesis). Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:882018

MLA 8th Edition

Ćurčić, Matea. "Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina." Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, 2016. https://urn.nsk.hr/urn:nbn:hr:163:882018

Chicago 17th Edition

Harvard

Ćurčić, M. (2016). 'Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina', Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, accessed 14 January 2025, https://urn.nsk.hr/urn:nbn:hr:163:882018

Vancouver

Ćurčić M. Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Pharmacy and Biochemistry; 2016 [cited 2025 January 14] Available at: https://urn.nsk.hr/urn:nbn:hr:163:882018

IEEE

M. Ćurčić, "Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina", Master's thesis, University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:163:882018

Cite this item: https://urn.nsk.hr/urn:nbn:hr:163:882018

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Title	Utjecaj antikoagulansa korištenog prilikom uzimanja uzoraka krvi na kromatografsku analizu N-glikana plazmatskih proteina
Title (english)	The impact of anticoagulant on chromatographic analysis of N-glycans released from plasma proteins
Author	Matea Ćurčić
Mentor	Olga Gornik (mentor)
Committee member	Olga Gornik (predsjednik povjerenstva)
Committee member	Nada Vrkić (član povjerenstva)
Committee member	Suzana Inić (član povjerenstva)
Granter	University of Zagreb Faculty of Pharmacy and Biochemistry (Department of biochemistry and molecular biology) Zagreb
Defense date and country	2016-07-15, Croatia
Scientific / art field, discipline and subdiscipline	BIOMEDICINE AND HEALTHCARE Pharmacy Medical Biochemistry
Abstract	Glikozilacija je najčešća i najvarijabilnija proteinska modifikacija koja ima važnu ulogu u usklađivanju različitih funkcija viših organizama. Glikani osim strukturne imaju i regulatornu ulogu te moduliraju mnoge biološke procese. Upravo zbog velike složenosti i strukturne raznolikosti glikani predstavljaju novu metu znanstvenih istraživanja koja kao uzorak često koriste humanu plazmu. Budući da je u takvim istraživanjima često teško kontrolirati predanalitičke postupke, cilj ovog rada je bio ispitati utjecaj uzimanja uzorka krvi korištenjem različitih antikoagulansa na rezultate analize Nglikozilacije plazmatskih proteina. Za ostvarivanje navedenog cilja prikupljeni su uzorci plazme ispitanika dobiveni vađenjem krvi na dva različita antikoagulansa, EDTA i Na-citrat. N-glikani su zatim otpušteni s proteina plazme enzimom PNGazom F i obilježeni 2-aminobenzamidom te analizirani tekućinskom kromatografijom visoke djelotvornosti koja se temelji na hidrofilnim interakcijama (HILIC-UPLC kromatografija). Dobiveni rezultati su pokazali da za 25 glikanskih skupina postoji statistički značajna razlika između mjerenja dobivenih nakon uzimanja uzoraka krvi na različiti antikoagulans. Dobivene razlike su male, ali dovoljne da upozore na važnost standardiziranog predanalitičkog postupka. Stoga, da bi interpretacija rezultata laboratorijskih istraživanja bila objektivna i točna, što se od nje i očekuje, nužno je razumijevanje i svjesnost o utjecaju različitih predanalitičkih čimbenika.
Abstract (english)	Glycosylation is the most common and variable protein modification which has important role in harmonizing the various functions of higher organisms. Other than structural, glycans have a regulatory role and modulate numerous biological processes. Due to the great complexity and structural diversity, glycans represent novel targets in scientific research. In this kind of research, it is often very difficult to control preanalytical factors. Therefore, the aim of this study was to examine the influence of anticoagulant used during blood sampling on the analysis of plasma proteins Nglycosylation. In order to achieve this goal, plasma samples were collected on two types of anticoagulant, EDTA and Na-citrate. Glycans were released using N-glycosidase F, labeled with 2-aminobenzamide and analyzed by high performance liquid chromatography based on hydrophilic interactions (HILICUPLC). Obtained results showed statistically significant difference for 25 glycan groups. Although those differences are small, they show the relevance of standardizing preanalytical procedure for glycan analysis. Therefore, in order to assure accurate interpretation of research results, it is necessary to understand the impact of preanalytical factors.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:163:882018
Study programme	Title: Medical biochemistry Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of Faculty of Pharmacy and Biochemistry University of Zagreb
Created on	2016-10-28 11:13:51