Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null

Lisica, Ana

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master's thesis

Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null

Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:523653

Lisica, Ana

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Lisica, A. (2009). Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:523653

MLA 8th Edition

Lisica, Ana. "Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:523653

Chicago 17th Edition

Harvard

Lisica, A. (2009). 'Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null', Master's thesis, University of Zagreb, Faculty of Science, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:217:523653

Vancouver

Lisica A. Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:217:523653

IEEE

A. Lisica, "Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:523653

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:523653

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Title	Prijenos proteina APP u organelima endocitoznog puta u stanicama CHO NPC1-null
Title (english)	Transport of APP protein in organelles of endocytic pathway in CHO NPC1-null cells
Author	Ana Lisica
Mentor	Silva Katušić Hećimović (mentor)
Mentor	Dubravka Hranilović (sumentor)
Committee member	Silva Katušić Hećimović (predsjednik povjerenstva)
Committee member	Dubravka Hranilović (član povjerenstva)
Committee member	Željka Vidaković Cifrek (član povjerenstva)
Committee member	Maja Matulić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2009, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Promijenjen metabolizam i povećana sinteza peptida amiloid β (Aβ) smatra se ključnim događajem u razvoju Alzheimerove bolesti (AB). Nedavna istraživanja su pokazala da razina kolesterola utječe na procesiranje prekursora proteina amiloid β (APP), te utječe na stvaranje i razinu peptida Aβ. Nakupljanje kolesterola i značajno povećanje razine peptida Aβ uočeno je i u Niemann Pickovoj bolesti tipa C (NPC), nasljednoj bolesti nakupljanja lipida, pa se ova bolest pokazala kao dobar model u proučavanju mehanizma djelovanja kolesterola na razvoj AB. Cilj rada je bio ispitati utječe li nakupljanje kolesterola u bolesti NPC na promijenjen smještaj proteina APP i presenilina 1 (PS1) u staničnim organelima endocitoznog puta. Korištenjem modelnih stanica CHO NPC1-/- i stanica divljeg tipa (CHOwt), pokazano je da stanice CHO NPC1-/- pokazuju promijenjen smještaj proteina APP u ranim i reciklirajućim endosomima. Stanice CHO NPC1-/- pokazuju promijenjeno bojanje biljega za rane i reciklirajuće endosome što ukazuje na njihovo nakupljanje na periferiji stanice. Međutim nije pokazan promijenjen smještaj proteina PS1 u organelima endocitoznog puta. Ovi rezultati ukazuju da bi kolesterol mogao djelovati na nastanak AB tako da regulira smještaj proteina APP unutar staničnih organela endocitoznog puta, te da takva preraspodjela proteina APP dovodi do povećanog stvaranja odsječka C99, te peptida Aβ u ovim organelima.
Abstract (english)	Altered metabolism and increased formation of amyloid-β peptide (Aβ) is considered to be the key event in development of Alzheimer's disease (AD). Recent reports have shown that cholesterol levels modulate processing of amyloid-β precursor protein (APP) and formation of Aβ peptide. Cholesterol accumulation and increased levels of Aβ peptide have also been observed in Niemann-Pick type C disease (NPC), hereditary sphingolipid storage disorder, which has become an important model to study the role of cholesterol in the pathogenesis of AD. The goal of this work was to investigate whether cholesterol accumulation in NPC disease changes distribution of APP protein and presenilin 1 (PS1) within endocytic pathway. Using immunoconfocal microscopy, we observed altered localization of APP protein in early and recycling endosomes in CHO NPC1-/- compared to CHOwt cells. In addition, we detected altered staining of markers for early (EEA1) and recycling (transferrin receptor, TFR) endosomes in CHO NPC1-/- cells, which suggests their accumulation on the cell periphery. However, altered localization of PS1 was not observed in endocytic organelles. These results suggest that cholesterol may influence the onset of AD by regulating trafficking of APP protein within endocytic pathway, and that this redistribution od APP protein leads to increased synthesis of C99, and Aβ peptide in these organelles.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:523653
Study programme	Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education Study programme type: university Study level: integrated undergraduate and graduate Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2018-03-13 11:06:13