Metabolizam proteina APP u lizosomskim bolestima nakupljanja

Posavec, Melanija

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master's thesis

Metabolizam proteina APP u lizosomskim bolestima nakupljanja

Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:840841

Posavec, Melanija

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Posavec, M. (2009). Metabolizam proteina APP u lizosomskim bolestima nakupljanja (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:840841

MLA 8th Edition

Posavec, Melanija. "Metabolizam proteina APP u lizosomskim bolestima nakupljanja." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:840841

Chicago 17th Edition

Posavec, Melanija. "Metabolizam proteina APP u lizosomskim bolestima nakupljanja." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:840841

Harvard

Posavec, M. (2009). 'Metabolizam proteina APP u lizosomskim bolestima nakupljanja', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:840841

Vancouver

Posavec M. Metabolizam proteina APP u lizosomskim bolestima nakupljanja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:840841

IEEE

M. Posavec, "Metabolizam proteina APP u lizosomskim bolestima nakupljanja", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:840841

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:840841

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Metadata

Title	Metabolizam proteina APP u lizosomskim bolestima nakupljanja
Title (english)	The metabolism of APP protein in lysosomal storage disorders
Author	Melanija Posavec
Mentor	Silva Katušić Hećimović (mentor)
Committee member	Dubravka Hranilović (predsjednik povjerenstva)
Committee member	Mirta Tkalec (član povjerenstva)
Committee member	Maja Matulić (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2009, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Nedavna istraživanja su pokazala da povišena razina kolesterola može mijenjati cijepanje prekursora proteina amiloid-beta (APP) i utjecati na nakupljanje peptida amiloid-beta (Aβ), glavne patološke značajke Alzheimerove bolesti (AB). Nakupljanje kolesterola u endosomima/lizosomima u bolesti Niemann-Pick tipa C (NPC) prati značajno povećanje razine peptida Aβ i C-terminalnog odsječka proteina APP - C99. Cilj rada je bio ispitati način djelovanja kolesterola na smještaj proteina APP i/ili presenilina 1 (PS1) u stanične organele u bolesti NPC. Uporabom modelnih stanica CHO NPC1-null i stanica divljeg tipa (CHOwt) u razdvajanju unutarstaničnih organela centrifugiranjem u gradijentu Iodixanola, utvrđeno je da stanice CHO NPC1-null pokazuju promijenjen smještaj proteina APP i PS1 te povišenu razinu kolesterola i odsječka C99 u ranim/kasnim endosomima. Ovi rezultati ukazuju da kolesterol najvjerojatnije doprinosi nastanku AB regulirajući smještaj proteina APP i PS1 unutar organela endocitoznog puta, središnjem mjestu stvaranja peptida Aβ u stanici, te da ova preraspodjela može dovesti do povećanog stvaranja peptida Aβ.
Abstract (english)	Recent reports have suggested that elevated cholesterol levels may modulate processing of beta-amyloid precursor protein (APP) by increasing formation of the amyloid-beta (Aβ) peptide, a central event in the pathogenesis of Alzheimer’s disease (AD). Cholesterol accumulation in endosomes/lysosomes in Niemann-Pick Type C (NPC) disease is accompanied by a significant increase in Aβ levels and levels of the Cterminal APP fragment – C99. The goal of this work was to investigate the cholesterol accumulation-regulated subcellular redistribution of APP and/or presenilin 1 (PS1) in NPC disease. To test this we utilized NPC model cells (CHO NPC1-null) and parental CHOwt cells. Using subcellular fractionation we observed altered subcellular localization of APP and PS1 together with increased C99 levels in early/late endosomes in CHO NPC1-null vs. CHOwt cells. Our findings suggest that cholesterol may contribute to AD onset by altering trafficking of APP and PS1 within endocytic pathway leading to increased formation of Aβ.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:840841
Study programme	Title: Molecular Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2018-03-15 10:41:04