Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)

Jezernik, Karlo

prikaz prve stranice dokumenta Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)

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master's thesis

Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)

Zagreb: University of Zagreb, Faculty of Science, 2014. urn:nbn:hr:217:198849

Jezernik, Karlo

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Jezernik, K. (2014). Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd) (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:198849

MLA 8th Edition

Jezernik, Karlo. "Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)." Master's thesis, University of Zagreb, Faculty of Science, 2014. https://urn.nsk.hr/urn:nbn:hr:217:198849

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Harvard

Jezernik, K. (2014). 'Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)', Master's thesis, University of Zagreb, Faculty of Science, accessed 23 September 2024, https://urn.nsk.hr/urn:nbn:hr:217:198849

Vancouver

Jezernik K. Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2014 [cited 2024 September 23] Available at: https://urn.nsk.hr/urn:nbn:hr:217:198849

IEEE

K. Jezernik, "Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:217:198849

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:198849

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Metadata

Title	Molekularna karakterizacija Coleus blumei viroida iz ukrasne koprive (Solenostemon scutellarioides (L.) Codd)
Title (english)	Molecular characterization of Coleus blumei viroids from coleus (Solenostemon scutellarioides (L.) Codd)
Author	Karlo Jezernik
Mentor	Dijana Škorić (mentor)
Committee member	Dijana Škorić (predsjednik povjerenstva)
Committee member	Željka Vidaković-Cifrek (član povjerenstva)
Committee member	Maja Matulić (član povjerenstva)
Committee member	Antun Alegro (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2014-10-24, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Viroidi su patogeni jednostavniji od virusa. Do sada su pronađeni jedino u biljkama gdje se mogu replicirati bez vidljivih štetnih posljedica po biljku ili mogu uzrokovati simptome slične virusnima. Njihov genom se sastoji od male (246-401 nt), nekodirajuće, jednolančane kovalentno zatvorene molekule RNA. Za rasprostranjivanje ne trebaju viruse pomagače i imaju mogućnost autonomne replikacije što ih čini najmanjim poznatim RNA-replikonima. Ukrasna kopriva (Solenostemon scutellarioides (L.) Codd) domaćin je viroidima roda Coleviroid koji dijele središnju očuvanu regiju (Central Conserved Region, CCR) i pripadaju porodici Pospiviroidae. Cilj mog rada bio je istražiti prisutnost viroida roda Coleviroid u odabranim uzorcima ukrasnih kopriva i identificirati pronađene viroidne vrste. Iz listova tri kultivara biljaka S. scutellarioides ekstrahirana je ukupna RNA. Na njoj je provedena reverzna transkripcija i lančana reakcija polimeraze (RT-PCR) s univerzalnim početnicama za rod Coleviroid dizajniranima prema središnjoj očuvanoj regiji. Amplikoni približnih veličina 250 i 360 baznih parova su klonirani i analizirani metodom SSCP (Single-strand Conformation Polymorphism). Najreprezentativniji produkti su sekvencirani i bioinformatički analizirani. Nukleotidni sljedovi su otkrili prisutnost CbVd-1 i CbVd-3 po prvi puta u Hrvatskoj.
Abstract (english)	Viroids are pathogens simpler than viruses found only in plants where they can replicate without adverse effects or can cause virus-like symptoms. Viroid genome comprises one small (246-401 nt), non-coding, single stranded covalently closed RNA molecule. Viroids do not depend on helper viruses for spreading and have autonomous replication which makes them the smallest known RNA-replicons. Solenostemon scutellarioides (L.) Codd hosts several viroids from genus Coleviroid that have common Central Conserved Region (CCR) and are members of the familiy Pospiviroidae. The aim of this work was to investigate the occurrence of Coleviroids in selected S. scutellarioides plants and to identify detected viroid species. Total RNA was extracted from leaf samples of three S. scutellarioides cultivars and subjected to reverse transcription and polymerase chain reaction (RT-PCR) with the use of universal primers designed specifically for Coleviroid CCR. Amplicons of about 250 and 360 base pairs were cloned and analyzed with SSCP (Single-strand Conformation Polymorphism) method. The most representative products were sequenced. Obtained sequences were analyzed and revealed the presence of two Coleviroides, namely, CbVd-1 and CbVd-3, for the first time in Croatia.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:198849
Study programme	Title: Molecular Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
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Repository	Repository of the Faculty of Science
Created on	2017-04-26 09:45:17