De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906

Boštjančić, Ljudevit Luka

prikaz prve stranice dokumenta De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906

master's thesis

De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906

Zagreb: University of Zagreb, Faculty of Science, 2021. urn:nbn:hr:217:429037

Boštjančić, Ljudevit Luka

University of Zagreb
Faculty of Science
Department of Biology

Cite this document

APA 6th Edition

Boštjančić, Lj. L. (2021). De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906 (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:429037

MLA 8th Edition

Boštjančić, Ljudevit Luka. "De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906." Master's thesis, University of Zagreb, Faculty of Science, 2021. https://urn.nsk.hr/urn:nbn:hr:217:429037

Chicago 17th Edition

Harvard

Boštjančić, Lj. L. (2021). 'De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906', Master's thesis, University of Zagreb, Faculty of Science, accessed 01 March 2025, https://urn.nsk.hr/urn:nbn:hr:217:429037

Vancouver

Boštjančić LjL. De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2021 [cited 2025 March 01] Available at: https://urn.nsk.hr/urn:nbn:hr:217:429037

IEEE

Lj. L. Boštjančić, "De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:217:429037

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:429037

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Title	De novo sastavljanje transkriptoma vrste Procambarus virginalis Lyko, 2017 i ekspresija gena u odgovoru na patogen Aphanomyces astaci Shikora, 1906
Title (english)	De novo assembly of Procambarus virginalis Lyko, 2017 transcriptome and gene expression in response to the pathogen Aphanomyces astaci Shikora, 1906
Author	Ljudevit Luka Boštjančić
Mentor	Kathrin Theissinger (mentor)
Mentor	Ivana Maguire (sumentor)
Committee member	Ivana Maguire (predsjednik povjerenstva)
Committee member	Marin Ježić (član povjerenstva)
Committee member	Rosa Karlić (član povjerenstva)
Committee member	Sandra Hudina (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2021-01-25, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Jedna on najveći prijetnji za populacije nativnih europskih slatkovodnih rakova je patogen Aphanomyces astaci Shikora, 1906 iz razreda Oomycetes, koji uzrokuje bolest račju kugu letalnu za nativne vrste slatkovodnih rakova. Mramorni rak, Procambarus virginalis Lyko, 2017 invazivna je vrsta i poznati prenositelj račje kuge. U ovom istraživanju proučen je imunosni odgovor 30 jedinki mramornog raka na infekciju s A. astaci. Korištena su dva soja patogena; PsI- visoko virulentan i As- nisko virulentan. Uzorkovanje hepatopankreasa rakova iz pokusa provedeno je tri i 21 dan nakon izlaganja patogenu te je provedena izolacija RNA. Iz fragmenata RNA, dobivenih sekvenciranjem, sastavljan je de novo transkriptom visoke kvalitete kojeg čini 60,004 gena. Za 41.9% transkripata je pronađena anotacija. Analiza diferencijalne ekspresije otkrila je 102 diferencijalno eksprimirana gena. Većina diferencijalno eksprimiranih gena bila je među uzorcima jedinki uzorkovanih 21 dana nakon infekcije. Među diferencijalno eksprimiranim genima bili su prisutni poznati efektori urođene imunosti rakova: serpin, lektini tipa-C i crustini. Novootkriveni su i geni s potencijalnom ulogom u urođenoj imunosti rakova. Oni predstavljaju predmet za buduća istraživanja urođene imunosti rakova
Abstract (english)	One of the biggest threats for freshwater crayfish populations is the pathogen Aphanomyces astaci Shikora, 1906 from the class Oomycetes that causes a disease lethal for native crayfish species, crayfish plague. The marbled crayfish, Procambarus virginalis Lyko, 2017 is an invasive crayfish species, known to be resistant to the crayfish plague disease. In this study the immune response to an A. astaci infection of 30 marbled crayfish exposed to two strains of A. astaci (PsI- high virulence and As- low virulence) was investigated. Hepatopancreas samples were taken at two time points, three days and 21 days post challenge. From the RNA sequencing reads, high-quality de novo transcriptomes of marbled crayfish were assembled containing 60,004 genes. Annotation was available for 41.9% of the transcripts. Differential gene expression analysis revealed in total 102 differentially expressed genes. More differentially expressed genes were observed among the samples from the second time point. Among the differentially expressed genes several known effectors of the crayfish innate immunity were detected. Previously unreported genes with potential role in crayfish innate immunity were also identified. They represent targets for future studies on crayfish innate immunity.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:429037
Study programme	Title: Molecular Biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access Embargo expiration date: 2023-01-25
Terms of use
Repository	Repository of the Faculty of Science
Created on	2021-01-28 11:20:00