Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1

Perić, Martina

prikaz prve stranice dokumenta Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1

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master's thesis

Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1

Split: University of Split, University of Split, Faculty of science, 2016. urn:nbn:hr:166:469247

Perić, Martina

University of Split
Faculty of Science
Department of Physics

Cite this document

APA 6th Edition

Perić, M. (2016). Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1 (Master's thesis). Split: University of Split, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:166:469247

MLA 8th Edition

Perić, Martina. "Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1." Master's thesis, University of Split, Faculty of Science, 2016. https://urn.nsk.hr/urn:nbn:hr:166:469247

Chicago 17th Edition

Harvard

Perić, M. (2016). 'Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1', Master's thesis, University of Split, Faculty of Science, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:166:469247

Vancouver

Perić M. Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1 [Master's thesis]. Split: University of Split, Faculty of Science; 2016 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:166:469247

IEEE

M. Perić, "Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1", Master's thesis, University of Split, Faculty of Science, Split, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:166:469247

Cite this item: https://urn.nsk.hr/urn:nbn:hr:166:469247

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Metadata

Title	Chaperones and metabolism in Saccharomyces cerevisiae: molecular docking of Hsp90 with Snf1 and Tor1
Title (croatian)	Šaperoni i metabolizam u pupajućem kvascu, Saccharomyces cerevisiae: modeliranje interakcije Hsp90 sa Snf1 i Tor1
Author	Martina Perić
Mentor	Paško Županović (mentor)
Committee member	Paško Županović (predsjednik povjerenstva)
Committee member	Anita Kriško (član povjerenstva)
Committee member	Jasna Puizina (član povjerenstva)
Granter	University of Split Faculty of Science (Department of Physics) Split
Defense date and country	2016-09-16, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Physics
Abstract	Aging is characterized by a progressive loss of physiological integrity and involves genetic pathways and biochemical processes that are conserved in evolution. The nine hallmarks of aging are not perceived as isolated phenomena but rather interconnected events, together building a complex regulatory network of signaling pathways. One of the most manageable and widely used models of cellular aging is the budding yeast (Saccharomyces cerevisiae). Communication between proteostasis and metabolic activity, in yeast as a model system, is in the spotlight of this thesis via the study of interactions between Hsp90, the main actor of protein maintenance, and Snf1 and Tor1, some of the main regulators of metabolic activity. Structural analysis of Hsp90, α subunit Snf1 of heterotrimeric complex SNF1 and Tor1 protein known to be involved in Saccharomyces cerevisiae metabolism was performed in silico by using dedicated programs. Combining experimentally obtained structures together with the modeling of protein-protein interactions known as docking, allowed us to build models of two protein complexes from the known structure of its constituents. Binding sites on Snf1 and Tor1 for protein-protein interaction with Hsp90 were predicted and described using softwares: Fpocket for protein cavity detection, ClusPro for protein-protein docking and visualization tools (PyMOL, Jmol and Bioblender).
Abstract (croatian)	Starenje je okarakterizirano progresivnim gubitkom fiziološkog integriteta i uključuje genetske puteve i biokemijske procese koji su evolucijski visoko konzervirani. Devet obilježja starenja se ne mogu percipirati kao izolirani fenomeni već radije kao međusobno povezani događaji koji zajedno grade kompleksnu regulatornu mrežu signalnih putova u organizmima. Pupajući kvasac (Saccharomyces cerevisiae) jedan je od najjednostavnijih i najčešće korištenih modela staničnog starenja. Komunikacija između proteostaze i metaboličke aktivnosti stanice kvasca je u srži ovog diplomskog rada. Strukturne analize proteina uključenih u očuvanje proteostaze i metabolizma kod S. cerevisiae, Hsp90, α podjedinice Snf1 heterotrimera SNF1 i Tor1, provedene su in silico pristupom. Kombinirajući eksperimentalno dobivene tercijarne strukture proteina s molekularnim modeliranjem protein-protein interakcija dobiven je model biomolekularnih kompleksa od interesa: Hsp90-Snf1 and Hsp90-Tor1. Vezna mjesta između gradivnih proteina u pojedinom kompleksu su predviđena i opisana uz pomoć računalnih programa i alata za vizualizaciju: Fpocket za identifikaciju potencijalnih veznih mjesta u proteinu, ClusPro za modeliranje protein-protein interakcije te alati za vizualizaciju proteinskih struktura (PyMOL, Jmol i Bioblender).
Keywords
Keywords (english)
Language	english
URN:NBN	urn:nbn:hr:166:469247
Study programme	Title: Physics; specializations in: major in teaching, major in Astrophysics, major in Biophysics, major in Enviromental physics, major in Computational physics Course: major in Biophysics Study programme type: university Study level: graduate Academic / professional title: magistar/magistra fizike (magistar/magistra fizike)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
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Repository	Repository of Faculty of Science
Created on	2017-07-07 15:46:48