prikaz prve stranice dokumenta Morphometric characteristics of erythrocytes in Lička pramenka sheep
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master's thesis
Morphometric characteristics of erythrocytes in Lička pramenka sheep
Zagreb: University of Zagreb, Faculty of Veterinary Medicine, 2024. urn:nbn:hr:178:465249
Lobpreis, Ingo Ralph Albin
University of Zagreb
Faculty of Veterinary Medicine

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Lobpreis, I. R. A. (2024). Morphometric characteristics of erythrocytes in Lička pramenka sheep (Master's thesis). Zagreb: University of Zagreb, Faculty of Veterinary Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:178:465249

Lobpreis, Ingo Ralph Albin. "Morphometric characteristics of erythrocytes in Lička pramenka sheep." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:178:465249

Lobpreis, Ingo Ralph Albin. "Morphometric characteristics of erythrocytes in Lička pramenka sheep." Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:178:465249

Lobpreis, I. R. A. (2024). 'Morphometric characteristics of erythrocytes in Lička pramenka sheep', Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, accessed 15 September 2024, https://urn.nsk.hr/urn:nbn:hr:178:465249

Lobpreis IRA. Morphometric characteristics of erythrocytes in Lička pramenka sheep [Master's thesis]. Zagreb: University of Zagreb, Faculty of Veterinary Medicine; 2024 [cited 2024 September 15] Available at: https://urn.nsk.hr/urn:nbn:hr:178:465249

I. R. A. Lobpreis, "Morphometric characteristics of erythrocytes in Lička pramenka sheep", Master's thesis, University of Zagreb, Faculty of Veterinary Medicine, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:178:465249

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Metadata
TitleMorphometric characteristics of erythrocytes in Lička pramenka sheep
Title (croatian)Morfometrijske osobitosti eritrocita ovaca pasmine lička pramenka
AuthorIngo Ralph Albin Lobpreis
MentorIvona Žura Žaja (mentor)
MentorBranimira Špoljarić (mentor)
Committee memberMaja Popović (predsjednik povjerenstva)
Committee memberIvona Žura Žaja (član povjerenstva)
Committee memberBranimira Špoljarić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Veterinary Medicine
Zagreb
Defense date and country2024-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Veterinary Medicine
Basic and Preclinical Veterinary Sciences
Abstract
To the best of our knowledge, there is no data in the recent literature on the analyses of ovine’s erythrocytes (RBCs) subpopulations regarding the morphometric size and shape parameters, using computer-assisted image analysis of RBC morphometry and multivariate statistical methods, including principal component and cluster analysis. The aims of this study are to determine the values of RBC haematological and morphometric size and shape parameters, to form RBC groups according to the obtained haematological parameters, to determine the differences in morphometric parameters between the formed RBC groups and to determine RBC subpopulations and their respective proportions in the formed groups. The 36 blood samples were collected from jugular vein of randomly chosen healthy sheep, 2 to 5 years old. Hematological parameters: haemoglobin (Hgb), haematocrit (HTC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and red blood cell distribution width (RDW) were analysed on hematology automized analyzer. Morphometric analyses were performed on 3600 RBCs in stained blood smears (n=36) by SFORM computer-assisted program and morphometric RBCs size and shape parameters were determined. Cluster analysis indicated three RBCs subpopulations based on the RBCs morphometric size and shape parameters (ES 1 - the smallest and most elongated RBCs; ES 2 - the biggest and most rounded RBCs; ES 3 - average size and shape RBCs). Differences in mean values of RBCs morphometric size and shape parameters between groups categorized by age and values of hematological parameters were established. Significantly higher proportion of ES 2 and ES 3 subpopulations and significantly lower proportion of ES 1 subpopulation was established in groups of RBCs with higher values of Hgb, HTC, MCV and MCH. These results could serve as a basis for the diagnostic interpretation of anemic syndromes in veterinary medicine especially concerning normocytic, macrocytic, and microcytic anemia in sheep.
Abstract (croatian)
Prema dostupnim podacima, ne postoje istraživanja koja su utvrdila subpopulacije eritrocita temeljem mofrometrijskih pokazatelja veličine i oblika, pomoću kompjuterski potpomognute morfometrijske analize eritrocita te multivarijantnih statističkih metoda, uključujući analize glavnih komponenti i klaster analize. Stoga su ciljevi ovog rada utvrditi vrijednosti hematoloških i morfometrijskih parametara veličine i oblika eritrocita, oblikovati skupine eritrocita prema dobivenim vrijednostima hematoloških parametara, utvrditi razlike u morfometrijskim parametrima između formiranih skupina eritrocita te odrediti subpopulacije eritrocita i njihov odnos u formiranim skupinama. Punkcijom vratne vene izvađena je krv 36 nasumično odabranih, zdravih ovaca u dobi 2 do 5 godina. Na automatiziranom hematološkom analizatoru određeni su: hemoglobin (Hgb), hematokrit (HTC), prosječni volumen eritrocita (MCV), prosječna količina hemoglobina u eritrocitu (MCH), prosječna koncentracija hemoglobina u krvi (MCHC) te širina distribucije eritrocita (RDW). Morfometrijskom analizom je izmjereno 3600 eritrocita pomoću SFORM računalnog programa na 36 krvnih razmaza na kojima su određivani morfometrijski pokazatelji veličine i oblika eritrocita. Na osnovi morfometrijskih pokazatelja veličine i oblika eritrocita klaster analizom su dobivene tri subpopulacije eritrocita (ES 1 – najmanji i najizduženiji eritrociti; ES 2 – najveći i najzaobljeniji eritrociti; ES 3 - eritrociti prosječnog oblika i veličine). Utvrđene su razlike u srednjim vrijednostima morfometrijskih pokazatelja veličine i oblika eritrocita između grupa kategoriziranih po dobi i vrijednostima hematoloških pokazatelja (više i niže vrijednosti Hgb, HCT, MCV, MCH, MCHC i RDW). Značajno viši udio subpopulacija ES 2 i ES 3, te niži ES 1 u grupama eritrocita utvrđen je s višim vrijednostima Hgb, HTC, MCV and MCH u odnosu na grupe s nižim vrijenostima istih pokazatelja. Dobiveni rezultati mogli bi doprinijeti preciznijoj dijagnostici anemija u veterinarskoj medicini, napose normocitnih, makrocitnih i mikrocitnih anemija u ovaca.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:178:465249
Study programmeTitle: Veterinary Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
RepositoryRepository of Faculty of Veterinary Medicine
Created on2024-06-17 11:12:31

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