Access restricted to students and staff of home institution
master's thesis
Utjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza

Mario Čuklić (2016)
Sveučilište u Zagrebu
Agronomski fakultet
Hranidba životinja
Metadata
TitleUtjecaj dodatka inokulata ili mliječne kiseline na proizvodnju amonijaka u silaži kukuruza
AuthorMario Čuklić
Mentor(s)Darko Grbeša (thesis advisor)
Abstract
Primarni cilj siliranja je očuvanje hranjivih tvari i energije koja se nalazi u zelenoj masi kroz duži vremenski period. Konzervirajući učinak postiže se uz pomoć anaerobnih uvjeta i djelovanja bakterija mliječne kiseline. Najnovija istraživanja ukazuju da unatoč niskom pH mikrobna aktivnost ustraje i u stabilnoj fazi, što bi za posljedicu moglo imati i kontinuirane promjene u dušićnim frakcijama. Cilj istraživanja bio je odrediti utjecaj dodatka inokulanta i mliječne kiseline prilikom siliranja cijele biljke kukuruza na sadržaj sirovih proteina (SP) i amonijaka (NH3) tijekom perioda od 364 dana stajanja silaže. Osim navedenih frakcija promatran je i utjecaj na sadržaj suhe tvari. Silaže su silirane s dodatkom inokulanta Bio-Sil koji u sebi sadrži lifilizirane kulture Lactobacillus plantarum 8862 i 8866 te s dodatkom mliječne kiseline u linearnom rasponu od 1 % do 7 % ST mase silaže. Sirovi protein određen je Kjeldahlovom metodom, a sadržaj amonijskog dušika spektrofotometrijski korištenjem Nesslerova reagensa. Mliječna kiselina i inokulant nisu značajno utjecali na sadržaj suhe tvari i sirovih proteina. Tijekom 364 dana bilježen je konstantan porast amonijaka u tretiranim i kontrolnim silažama što je potvrda porasta proteolize i deaminacije u ispitivanim silažama. Najveći porast vrijednosti amonijaka zabilježen je u kontrolnim silažama (0−6,69 % SP), dok je proizvodnja amonijaka bila najmanja u silažama tretiranim mliječnom kiselinom (0−4,23 % SP). Kod silaža tretiranih inokulantom zabilježen je porast amonijaka od 0 do 6,29 % SP. Dodatak inokulanta smanjio je obujam proteolize i deaminacije što je najvjerojatnije uvjetovano dominacijom Lactobacillus plantaruma u takvim silažama. Dodatak mliječne kiseline umanjio je opću mikrobnu aktivnost što je rezultiralo smanjenjem ukupnih proteolitičkih aktivnosti i redukcijom sinteze amonijaka.
KeywordsWhole-plant corn silage ammonia silage inoculant lactic acid proteolysis
Committee MembersDarko Grbeša (committee chairperson)
Goran Kiš (committee member)
Zlatko Svečnjak (committee member)
Marija Duvnjak (committee member)
GranterSveučilište u Zagrebu
Agronomski fakultet
Lower level organizational unitsHranidba životinja
PlaceZagreb
StateCroatia
Scientific field, discipline, subdisciplineBIOTECHNICAL SCIENCES
Agronomy
Study programme typeuniversity
Study levelgraduate
Study programmeAnimal Nutrition and Feed Science
Academic title abbreviationmag. ing. agr.
Genremaster's thesis
Language Croatian
Defense date2016-09-22
Parallel abstract (English)
Ensiling is a method of preservation nutrients and energy located in forage for longer time periods. Preservative effect is achieved by establishing anaerobic conditions and by lactic acid bacteria activity in ensiled material. The latest studies indicate that despite low pH microbial activity persists in a stable phase. This activity can result in a continuous changes in nitrogen fractions. The subject of this study was to determine the influence of inoculant and lactic acid bacteria on crude protein (CP), ammonia (NH3) and dry matter (DM) content in whole plant corn silage during ensiling period of 364 days. Silages were ensiled with the addition of Bio-Sil inoculant which contains liofilisated cultures of Lactobacillus plantarum 8862 and 8866, and with the addition of lactic acid in the linear range from 1% to 7% DM silage mass. CP was determined by the Kjeldahl method and ammonium nitrogen by spectrophotometry using Nessler reagent. Lactic acid and inoculant did not significantly affect DM and CP. During 364 days a constant increase in ammonia in treated and control silages was recorded and is confirmation of an increase in proteolysis and deamination in tested silages. The highest ammonia level was recorded in control silages (0 to 6.69%of CP), while the production of ammonia was smallest in silages treated with lactic acid (0 to 4.23% of CP). In silages treated with inoculant ammonia content increased from 0 to 6.29% of CP. Inoculation reduced proteolysis and deamination extent possibly influenced by the domination of Lactobacillus plantarum in these silages. Addition of lactic acid reduced the overall microbial activity, resulting in a decrease of total proteolytic activity and reduction of ammonia synthesis.
Parallel keywords (Croatian)kukuruzna silaža amonijak inokulant mliječna kiselina proteoliza
Resource typetext
Access conditionAccess restricted to students and staff of home institution
Terms of usehttp://rightsstatements.org/vocab/InC/1.0/
URN:NBNhttps://urn.nsk.hr/urn:nbn:hr:204:739674
CommitterValentina Bezek