Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela

Žučko, Jurica

prikaz prve stranice dokumenta Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela

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master's thesis

Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2005. urn:nbn:hr:159:054195

Žučko, Jurica

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

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APA 6th Edition

Žučko, J. (2005). Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:054195

MLA 8th Edition

Žučko, Jurica. "Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2005. https://urn.nsk.hr/urn:nbn:hr:159:054195

Chicago 17th Edition

Harvard

Žučko, J. (2005). 'Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:054195

Vancouver

Žučko J. Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2005 [cited 2025 February 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:054195

IEEE

J. Žučko, "Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2005. Available at: https://urn.nsk.hr/urn:nbn:hr:159:054195

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:054195

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Title	Predviđanje funkcija domena poliketidnih sintetaza pomoću skrivenih Markovljevih modela
Title (english)	Function prediction of polyketide synthase domains using hidden Markov models
Author	Jurica Žučko
Mentor	Daslav Hranueli (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Section for Bioinformatics) Zagreb
Defense date and country	2005-06-13, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Poliketidi su jedna od najčešće upotrebljavanih vrsta spojeva za dobivanje novih, biološki aktivnih supstancija. Razlog tome je način njihove biosinteze, koja se odvija u stupnjevima, pri čemu se u svakom stupnju poliketidni lanac produži za dva ugljikova atoma. Za dobivanje novih spojeva najčešće se upotrebljava postupak kombinatorne biosinteze, dodavanjem, uklanjanjem ili zamjenom domena ili modula poliketidnih sintetaza. Tim postupkom su dobiveni novi poliketidni spojevi, ali je njihov prinos u pravilu nizak. Kako taj problem nije razriješen, razvijen je novi postupak za dobivanje novih poliketida – mjesno-specifična mutageneza. Zamjenom pojedinih aminokiselina AT domena uspjela se promijeniti njihova specifičnost za supstrat. Matematički model, testiran u ovom radu, traži unutar višestrukog poravnanja domena stupce koji bi mogli imati važnu ulogu u određivanju funkcije domene. Zamjenom aminokiselina na tim pozicijama mogla bi se promijeniti funkcija domene.
Abstract (english)	The polyketide natural products are a group of chemical compounds most frequently used to obtain new biologically active substances. The reason for that is their stepwise way of biosynthesis, where in each step the polyketide chain grows by two carbon atoms. Combinatorial biosynthesis is mostly used for obtaining new polyketides by adding, deleting or swapping domains or modules of the polyketide synthases. Numerous new compounds were synthesised using combinatorial biosynthesis, but the product titre was too low for commercial production. Since this problem has not been solved yet, a new approach using site-specific mutagenesis was developed. Until now the change of AT domain substrate specificity was achieved by substituting a few amino acids. The mathematical model tested in this work searches within multiple alignment for columns that might play an important role in determining the function of the domain. Changing the amino acids on those positions should alter the function of the domain.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:054195
Study programme	Title: Biotechnology; specializations in: Biochemical Engineering Course: Biochemical Engineering Study programme type: university Study level: graduate Academic / professional title: diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo (diplomirani inženjer biotehnologije; smjer biokemijsko inženjerstvo)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2017-09-13 09:27:41