Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije

Cavrić, Marija

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master's thesis

Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:786316

Cavrić, Marija

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

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APA 6th Edition

Cavrić, M. (2021). Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:786316

MLA 8th Edition

Cavrić, Marija. "Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:786316

Chicago 17th Edition

Harvard

Cavrić, M. (2021). 'Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 09 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:786316

Vancouver

Cavrić M. Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 July 09] Available at: https://urn.nsk.hr/urn:nbn:hr:159:786316

IEEE

M. Cavrić, "Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:786316

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:786316

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Title	Učinak hidrolizata proteina uljne pogače sjemenki lana na rast, produktivnost i metabolizam CHO DP-12 stanične linije
Title (english)	Effect of flaxseed oil cake protein hydrolysates on growth, productivity and metabolism of CHO DP-12 cell line
Author	Marija Cavrić
Mentor	Igor Slivac (mentor)
Committee member	Višnja Gaurina-Srček (član povjerenstva)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Cell Technology, Application and Biotransformations) Zagreb
Defense date and country	2021-09-24, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Životinjski serum je standardni dodatak mediju za uzgoj životinjskih stanica. Međutim, zbog određenih nedostataka već više od tri desetljeća istražuje se upotreba biljnih hidrolizata proteina kao zamjene serumu u biotehnološkim postupcima proizvodnje rekombinantnih proteina pomoću životinjskih stanica. Cilj ovog rada bio je utvrditi utjecaj hidrolizata proteina uljne pogače sjemenki lana, dobivenih pomoću proteolitičkih enzima Alcalase, Neutrase i Protamex, na rast, produktivnost i metabolizam CHO DP-12 stanične linije. Osim utjecaja hidrolizata ukupnog sadržaja peptida, ispitan je i utjecaj peptidnih frakcija veličine do 10 i do 1 kDa kao dodataka mediju za uzgoj u koncentraciji od 2 i 0.5 g Lˉ¹ . Rezultati su pokazali kako dodani hidrolizati i njihove frakcije nemaju značajan utjecaj na rast stanica, dok je pokazan pozitivan utjecaj na produktivnost. Dodatak hidrolizata dobivenog pomoću enzima Protamex u koncentraciji 2 g Lˉ¹ rezultirao je visokom produktivnošću dok je istovremeno usporio rast stanica.
Abstract (english)	Animal serum is a standard media supplement for animal cultures, but because of its disadvantages, for more than three decades, the use of plant protein hydrolysates as a serum replacement in biotechnological processes for the production of recombinant proteins with cell cultures is researched. This study aimed to determine the influence of flaxseed oil cake protein hydrolysates, obtained by proteolytic enzymes, Alcalase, Neutrase and Protamex, on growth, productivity and metabolism of CHO DP-12 cell line. In addition to the influence of hydrolysates of the total peptide content, fractions of peptides size up to 10 kDa and 1 kDa were tested as culture media supplements in concentrations of 2 and 0.5 g L-1. Research showed that the added hydrolysates and their fractions did not have a significant impact on cell growth while a positive effect on productivity was shown. The addition of hydrolysate obtained with Protamex in a concentration of 2 g Lˉ¹ resulted in high productivity while at the same time slowing down cell growth.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:786316
Study programme	Title: Molecular Biotechnology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
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Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2021-10-27 11:55:59