Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin

Krpan, Maja

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master's thesis

Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:875374

Krpan, Maja

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Fermentation and Yeast Technology

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APA 6th Edition

Krpan, M. (2012). Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:875374

MLA 8th Edition

Krpan, Maja. "Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:875374

Chicago 17th Edition

Harvard

Krpan, M. (2012). 'Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 08 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:875374

Vancouver

Krpan M. Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2025 February 08] Available at: https://urn.nsk.hr/urn:nbn:hr:159:875374

IEEE

M. Krpan, "Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:875374

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:875374

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Metadata

Title	Izolacija mutanata kvasca Pichia stipitis rezistentnih na nistatin
Title (english)	Isolation nystatin -resistant mutants of yeast Pichia stipitis
Author	Maja Krpan
Mentor	Damir Stanzer (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Food Engineering) (Laboratory for Fermentation and Yeast Technology) Zagreb
Defense date and country	2012-04-11, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	(S)-adenozil-L-metionin (SAMe) je važna molekula u metabolizmu svih živih stanica. Nedostatci kao i povećane količine SAMe u organizmu mogu uzrokovati razne poremećaje u organizmu, a terapija SAMe-om je prisutna u liječenju bolesti jetre, depresije i Alzheimerove bolesti. Stoga je od iznimne važosti pronaći što učinkovitije i jeftinije načine proizvodnje. Cilj ovog rada bio je mutirati divlji soj kvasca Pichia stipitis UV zračenjem, ispitati njegovo preživljavanje prilikom navedenog zračenja i pronaći UV-mutanta sa što većom rezistencijom na antibiotik nistatin i sa smanjenon tolerancijom na EtOH odnosno UVmutanta koji svojim metabolizmom proizvodi veću količinu SAMe nego divlji soj. Rezultati pokazuju da je doza zračenja od 120 J/m2 prikladna za proizvodnju mutanata UV-zračenjem, proizvedeni mutanti toleriraju veće koncentracije nistatina od divljeg soja te pokazuju različitu toleranciju na etanol.
Abstract (english)	(S)-Adenosyl-L-methionine (SAMe) is an important molecule in metabolism of all living cells. Absence and extortionate amount of this substance can cause different disfunctions in organism. SAMe is used in treatment of liver disease, depression and Alzheimer´s disease. Therefore it is very important to find more efficient ways of producing SAMe. The aim of this work was to apply UV irradiation on yeast Pichia stipitis, to examine its capability to survive and acquire yeast with better resistance to antibiotic nystatin and lower tolerance to ethanol, yeast that produce more SAMe. The results showed that the amount of 120 J/m2 UV irradiation is adequate for producing these mutants. Mutants of yeast tolerate higher concentrations of nystatin compared to unmutated yeast and show different tolerance to ethanol.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:875374
Study programme	Title: Bioprocess Engineering Study programme type: university Study level: graduate Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resource	Text
File origin	Born digital
Access conditions	Access restricted to students and staff of home institution
Terms of use
Repository	Repository of the Faculty of Food Technology and Biotechnology
Created on	2017-05-08 10:26:23