Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula

Šimatović, Ana

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master's thesis

Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula

Zagreb: University of Zagreb, Faculty of Science, 2009. urn:nbn:hr:217:933768

Šimatović, Ana

University of Zagreb
Faculty of Science
Department of Biology

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APA 6th Edition

Šimatović, A. (2009). Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula (Master's thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:933768

MLA 8th Edition

Šimatović, Ana. "Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula." Master's thesis, University of Zagreb, Faculty of Science, 2009. https://urn.nsk.hr/urn:nbn:hr:217:933768

Chicago 17th Edition

Harvard

Šimatović, A. (2009). 'Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula', Master's thesis, University of Zagreb, Faculty of Science, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:217:933768

Vancouver

Šimatović A. Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula [Master's thesis]. Zagreb: University of Zagreb, Faculty of Science; 2009 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:217:933768

IEEE

A. Šimatović, "Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula", Master's thesis, University of Zagreb, Faculty of Science, Zagreb, 2009. Available at: https://urn.nsk.hr/urn:nbn:hr:217:933768

Cite this item: https://urn.nsk.hr/urn:nbn:hr:217:933768

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Title	Struktura i ekspresija gena za proteine Rad51 i Rad51D iz morske spužve Suberites domuncula
Title (english)	Structure and expression of genes for proteins Rad51 and Rad51D from marine sponge Suberites domuncula
Author	Ana Šimatović
Mentor	Helena Ćetković (mentor)
Committee member	Mirjana Kalafatić (predsjednik povjerenstva)
Committee member	Maja Matulić (član povjerenstva)
Committee member	Željka Vidaković Cifrek (član povjerenstva)
Granter	University of Zagreb Faculty of Science (Department of Biology) Zagreb
Defense date and country	2009, Croatia
Scientific / art field, discipline and subdiscipline	NATURAL SCIENCES Biology
Abstract	Proteini od interesa, Rad51 i Rad51D, imaju vrlo važnu ulogu kod sisavaca u održavanju integriteta genoma. Postojanje ovih proteina utvrđeno je i kod drugih kralježnjaka, te kod beskralježnjaka. Modelni organizam, morska spužva Suberites domuncula pripada koljenu Porifera, najstarijim danas živućim mnogostaničnim organizmima, i stoga je vrlo zanimljiva za istraživanje evolucijski sačuvanih gena/proteina. Metodama umnožavanja DNA lančanom reakcijom polimeraze i određivanja slijeda nukleotida u molekuli DNA Sangerovom dideoksi metodom utvrđen je nukleotidni slijed gena rad51 i rad51D te položaj introna. Pomoću algoritma BLAST i programa Clustal X i GeneDoc uspoređene su dobivene proteinske sekvence s njihovim ortolozima u drugim organizmima. Proteinske sekvence i položaj introna očekivano pokazuju veću homologiju s ortolozima iz kralježnjaka, nego iz beskralježnjaka. Gen rad51D ukloniran je u ekspresijski vektor kojim su transformirane stanice BL21. Nedovoljna koncentracija proteina se zadržava u topljivoj fazi, većina odlazi u talog gdje se agregira u obliku inkluzijskih tijela.
Abstract (english)	The proteins of interest, Rad51 and Rad51D play a very important part in maintaining mammalian genome integrity. The existence of these proteins was detected in other vertebrates, as well as in invertebrates. The model organism, marine sponge Suberites domuncula belongs to the phylum Porifera, the oldest living Metazoa. Therefore, the study of its genes/proteins that have been conserved through evolution is very interesting. Polymerase chaine reaction for multiplying DNA and Sanger dideoxynucleotide sequencing method were used in order to determine the nucleotide sequence for genes rad51 and rad51D and their intron position. Using BLAST searches of GenBank database and Clustal X and GeneDoc programmes, the obtained protein sequences were compared to their orthologs in other organisms. As expected, the protein sequences and intron position show more homology with their vertebrate orthologs than with their invertebrate orthologs. The rad51D gene was cloned into the expression vector which was used to transform BL21 cells. Insufficient protein concentration is contained in the supernatant, due to the fact that most proteins sediment and aggregate to form inclusion bodies.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:217:933768
Study programme	Title: Biology; specializations in: Molecular biology Course: Molecular biology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra biologije (magistar/magistra biologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Repository	Repository of the Faculty of Science
Created on	2018-03-21 09:33:37