Honey is one of highly nutritive value food known from ancient times. It is mainly composed of carbohydrates (of which fructose and glucose for nearly 85 – 95 %) and other minor substances, such as organic acids, amino acids, proteins, minerals vitamins and lipids. The quality of honey is mainly determined by its sensorial, chemical, physical and microbiological properties. The main purpose of this research was to evaluate microbiological properties of 72 honey samples and to evaluate number and/or presence of aerobic mesophilic and sporogenic bacteria, moulds and yeasts, sulphite-reducing clostridia, family of Enterobacteriaceae and specie of Staphylococcus aureus. Microbiological quality of all samples was considered good and all samples showed to be negative in respect to safety parameters. Inhibitory potential of selected honey samples was also investigated. Of tested honey concentrations (0,1 %, 5 %, 10 %, 25 %, 50 % i 75 %), final concentration of 75 % was the most potent. Honeydew and chestnut honey exhibited the most potent inhibitory effect to tested bacterial species, while the lowest inhibition exhibited lime (linden) honey. Among the same honey specie, considerable different inhibitory activity can be detected. The most sensitive bacterial specie was Staphylococcus aureus while the most resistant one was Enterococcus faecalis.